Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

CD43

The CD43 antigen is a 95-135 kDa heavily O-glycosylated and sialylated single chain membrane glycoprotein. This cell surface mucin-type glycoprotein is also called leukosialin or sialophorin. CD43 antigen is expressed by T lymphocytes, plasma cells, neutrophils, weakly on resting B lymphocytes but highly after activation, and on platelets. A soluble form of CD43 (galactoglycoprotein) can be detected in plasma. This form is produced by proteolytic shedding of the extracellular portion of the membrane molecule after cell activation. Studies, coupled with recent evidence that CD43 influences cellular adhesion, indicate that differential expression of CD43 may play a role in monocyte/macrophage trafficking.

Lysed normal whole blood sample

Clone: DFT1 Isotype: IgG1 Mouse The DFT1 antibody reacts with an extracellular epitope of the CD43 antigen. It is useful for detecting leucocytes by flow cytometry and immunohistochemistry on routinely-fixed and parrafin-embedded tissue sections. The DFT1 antibody is also able to induce homotypic adhesion of leucocytes.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM3264U A32560 A89307

2 mL 2 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR

IOTest IOTest IOTest IOTest

GMP GMP GMP

PE

APC-A750 APC-A750

0.5 mL 50 tests

B49195

CE

98/79/EC

The CD44 is a transmembrane glycoprotein of 85-200 KDa. Multiple CD44 isoforms have been described. CD44 is present on most cells or tissues, but not on platelets, hepatocytes, cardiac muscle, kidney tubular epithelium, testis and skin portions. CD44 is a signaling platform that integrates cellular microenvironmental cues with growth factor and cytokine signals. It transduces signals to membrane-associated cytoskeletal proteins or to the nucleus to regulate a variety of gene expression levels related to cell-matrix adhesion, cell migration, proliferation, differentiation, and survival. This cell adhesion molecule plays an important role in tumor progression and metastasis. Its role in tumorigenesis is due to its binding to extracellular matrix components, including hyaluronan (HA) and osteopontin (OPN), and to messenger molecules, such as growth factors present in the tumor microenvironment. The human Indian (In) blood group antigens a/b reside on CD44. CD44 Isotype: IgG1 Mouse J.173 antibody does not inhibit the binding of hyaluronate to its receptor. It can trigger IL-2-dependent proliferation and cytotoxicity of human T cell clones in vitro . Clone: J.173

Lysed normal whole blood sample.

Form

Size

Format

Part#

Status

Line Quality Standard

PB

B37789 IM1219U A32537 B30637

0.5 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

GMP GMP GMP GMP

FITC

2 mL 2 mL

PE

APC-A750

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 61 Outside US Flow Cytometry Catalog