Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD42a antigen (GPIX) is a single-chain transmembrane glycoprotein of 22 kDa that forms a noncovalent complex with CD42b, CD42c and CD42d (GPIb α , GPIb β , and GPV). The CD42a expression is restricted to platelets and megakaryocytes. The GPIb-IX-V complex is the platelet receptor for von Willebrand factor and is known as von Willebrand's factor-dependent adhesion receptor. Although GPIb-IX-V primarily functions as a platelet receptor for von Willebrand factor (VWF), it can also bind to other ligands present in circulation such as thrombin, P-selectin, integrin α M β 2, factor XI, factor XII, high- molecular-weight kininogen, as well as a number of snake venom proteins. CD42a

Platelet rich plasma from a normal whole blood sample.

Clone: SZ1 Isotype: IgG2a Mouse The SZ1 monoclonal antibody reacts with the CD42a-d complex but does not recognize GPIb or GPIX individually.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1757U IM0538

2 mL

Liquid

ASR RUO

IOTest

GMP GMP

UNLB

0.2 mg

Freeze-dried

-

The CD42b antigen (GPIb α ) is a membrane glycoprotein of 135 kDa, that forms a disulfide linked 160 kDa membrane protein with the CD42c (GP1b β ) known as GP1b. GP1b forms a noncovalent complex with GPIX (CD42a) and GPV (CD42d). This antigen is found on platelets and megakaryocytes. It has also been reported on vascular and tonsillar endothelial cells. The CD42 complex serves as the von Willebrand Factor(vWF) surface receptor involved in the adhesion of platelets to the subendothelium of damaged vascular walls. CD42b

Platelet rich plasma (PRP) from a normal whole blood sample.

Clone: SZ2 Isotype: IgG1 Mouse The SZ2 monoclonal antibody prevents the ristocetin-dependent binding of von Willebrand's factor to platelets and inhibits ristocetin-induced platelet agglutination. Platelet aggregation induced by type I collagen and Platelet Activating Factor (PAF) is also inhibited. The SZ2 antibody immunoprecipitates the components of the GPIb complex. It may also be used in Western blot.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM0648U IM1417U B13980 IM0409

2 mL 2 mL

Liquid Liquid Liquid

ASR ASR ASR RUO

IOTest IOTest IOTest

GMP GMP GMP GMP

PE

APC

0.5 mL 0.2 mg

UNLB

Freeze-dried

-

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 60 Outside US Flow Cytometry Catalog