Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD39 molecule, known as ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1), is a 70-100 kDa integral membrane glycoprotein with two transmembrane domains. It belongs to the family of the primary enzymes responsible for cell surface nucleotide hydrolysis, with ATPase and ADPase activity. CD39 antigen is expressed on activated lymphocytes, regulatory T cells, B cells, and dendritic cells. It is involved in control of the extracellular nucleoside triphosphate pool (NTP), suppression of inflammation and control of platelet activation. The coordinated expression of CD39/CD73 on Tregs and the adenosine A2A receptor on activated T effector cells generates immunosuppressive loops. In human studies, it has been reported that CD4+CD25-CD39+ T cells are T inducers. CD39

Lysed normal whole blood sample.

Form Clone: BA54

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

B55384 B55385

1 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

PC5.5

0.5 mL

The CD40 antigen is a 44-48 kDa type I integral membrane protein of the tumor necrosis factor receptor (TNFR) superfamily. This antigen is found on B cell lines, is strongly expressed by interdigitating cells (IDC), basal epithelial cells, and is also present on macrophages, some endothelial cells, and follicular dendritic cells. It is a pan-B marker, absent only from terminally differentiated plasma cells. CD40 is implicated in the process of B cell selection in the germinal centre. Studies demonstrated that CD40 monoclonal antibodies (mAbs) induce strong homotypic adhesions in resting B cells and, together with interleukin-4 (IL-4) maintain the cell cycle of blasts of the B lineage. They also can promote the switch to IgE secretion. Activated B cells via CD40 antigen in the presence of IL-10 differentiate into plasma cells and secrete large amounts of immunoglobulins. The CD154, (CD40 ligand) is a membrane glycoprotein on activated T cells that induces B cell proliferation and immunoglobulin secretion. CD154 is also expressed on activated platelets and triggers an inflammatory reaction of endothelial cells. CD40

Lysed normal whole blood sample.

Clone: MAB89

Isotype: IgG1 Mouse

Co-culture with MAB89 and IL-4 leads to long term B cell proliferation.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM1936U B20025 B30636

2 mL

Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest

GMP GMP GMP GMP

PC5.5

0.5 mL 0.5 mL 0.2 mg

APC

UNLB

IM1374

Freeze-dried

-

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 58 Outside US Flow Cytometry Catalog