Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD34 antigen is a monomeric transmembrane phosphoglycoprotein of about 110 kDa with two distinct extracellular domains. The membrane proximal domain, of about 110 amino acids, probably adopts a globular conformation. The NH2-terminal domain, of about 140 amino acids, is heavily glycosylated with both N-linked glycans and sialylated O-linked carbohydrates and probably exhibits an extended rod-like structure typical of mucin-like glycoproteins. The CD34 antigen is expressed on hematopoietic progenitor cells of all lineages as well as the most primitive pluripotential stem cells. CD34 antigen expression is highest on the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. Variations of glycosylation are thought to occur during normal hematopoiesis depending on lineage commitment and the level of cellular maturation. CD34

KG1A cell line

Clone: Immu133

Isotype: IgG1 Mouse

Epitope Class I : Immu133 monoclonal antibody detects sialic acid-dependent class I epitope.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM1420

100 tests

Liquid

RUO

IOTest

GMP

Clone: QBEnd10 Isotype: IgG1 Mouse Epitope Class II: QBEnd10 reacts with early normal hematopoietic progenitor cells, and with vascular endothelial cells.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM1250U IM2650U IM0786

2 mL 1 mL

Liquid Liquid

ASR ASR RUO

IOTest IOTest

GMP GMP GMP

PC5

UNLB

0.2 mg

Freeze-dried

-

Clone: 581

Isotype: IgG1 Mouse

Epitope Class III: Clone 581 recognizes a sialidase / glycoprotease / chymopapain insensitive class III epitope.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC FITC

IM1870

100 tests

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid

CE

IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest

98/79/EC

IM1870U A07776 IM1871U IM2709U B49202 A07777 IM2648U A51077 A21691 IM2472 IM2472U A86354 B92417 A89309 B92463

2 mL

ASR

GMP

PE PE

100 tests

CE

98/79/EC

2 mL 1 mL

ASR ASR

GMP GMP

ECD ECD PC5 PC5 PC7 PC7 APC APC

100 tests 100 tests

CE CE

98/79/EC 98/79/EC

1 mL 1 mL

ASR ASR

GMP GMP

100 tests 100 tests

CE CE

98/79/EC 98/79/EC

1 mL

ASR ASR

GMP GMP

APC-A700 APC-A700 APC-A750 APC-A750

0.5 mL 50 tests 0.5 mL 50 tests

CE

98/79/EC

ASR

GMP

CE

98/79/EC

Clone: QBEnd10 + Immu133 + Immu409 Isotype: IgG Mouse The CD34 Pool Kit includes two vials. The one labeled CD34 Pool-PE contains a mixture of QBEnd10, Immu133 and Immu409. The one labeled Isotypic Control IgG(1+2a)-PE is its matching negative control. As QBEnd10 and Immu133 are of the mouse IgG1 isotype and Immu409 of the IgG2a isotype, this control is a mixture of two R-phycoerythrin-conjugated irrelevant mouse monoclonal immunoglobulins of these isotypes. Each isotype concentration in the control matches the total concentration of each isotype in the CD34 Pool.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM1459U

2 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 55 Outside US Flow Cytometry Catalog