Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD27 molecule is a transmembrane disulfide-linked homodimer which belongs to tumor necrosis factor receptor (TNFR) / nerve growth factor receptor (NGFR) family. The molecular weight of the recognized antigen is 55 kDa / 120 kDa under reducing / non reducing conditions respectively. The CD27 glycoprotein is found on medullary thymocytes, peripheral T cells, subset of mature B cells and NK cells. On T cells, CD27 is preferentially expressed on the CD45RA+ CD45RO- naive subset of CD4+ T lymphocytes whereas most memory T cells (CD45RA- CD45RO+) lack CD27. Activation of T cells, when involving CD27 molecule, results in the upregulation of CD27 expression as cell surface embedded, and also in the release of a soluble form of CD27 (sCD27). CD70 antigen, which is a member of the TNF ligand superfamily, interacts with CD27 molecule and is known to be the CD27-ligand. CD27

Lysed normal whole blood sample.

Form Clone: 1A4CD27

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PB PE PE

B30635 IM2578 B96790 B26603 6607107 B21444 A54823 B49205 B09983

0.5 mL

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid

ASR ASR

IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest

GMP GMP

2 mL

100 tests

CE

98/79/EC

ECD PC5

0.5 mL

ASR RUO ASR ASR

GMP GMP GMP GMP

100 tests

PC5.5

0.5 mL

PC7 PC7 APC

1 mL

100 tests

CE

98/79/EC

0.5 mL 0.5 mL

ASR ASR

GMP GMP

APC-A750

B12701

The CD28 antigen (Tp44) is a homodimer of disulfide-linked chains, with a molecular weight of 44 kDa each. It is involved in the interaction of T lymphocytes with antigen-presenting cells (APCs), through its counter-receptors, B7-1/BB-1 (CD80) and B7-2/B70 (CD86). It provides a major co-stimulatory signal for T cell activation, proliferation and lymphokine production. The CD28 family of receptors (CD28, CTLA-4, ICOS, PD-1 and BTLA) plays a critical role in controlling the adaptive immune response. The CD28 receptor can enhance T cell antigen receptor (TCR) signals, as well as deliver independent signals. Although the signals through CD28 are crucial for the initial co-stimulation of interleukin-2 (IL-2) production, a TCR- independent CD28 signal leads to the selective transcription of survival, but not proliferative genes. CD28 is expressed by a majority of CD3+ T lymphocytes. CD4+ T lymphocytes express the CD28 antigen more frequently than do CD8+ T lymphocytes. CD28 antigen is also present on plasma cells and thymocytes. CD28 Isotype: IgG1 Mouse The CD28.2 monoclonal antibody costimulates T cell proliferation and cytokine production in the presence of T cell activators ( α CD3, PMA, SEB). It inhibits proliferation of CD4 + T lymphocytes. It cross-reacts with Rhesus monkey peripheral blood lymphocytes. Clone: CD28.2

Lysed normal whole blood sample.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1236U IM2071U 6607111 6607108 B24027 B23313 B10244 B12696 B08757 IM1376

2 mL 2 mL

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid

ASR ASR RUO RUO ASR ASR ASR ASR ASR ASR

IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest

GMP GMP GMP GMP GMP GMP GMP GMP GMP GMP

PE

ECD PC5

100 tests 100 tests

PC5.5

0.5 mL 0.5 mL 0.5 mL 0.5 mL 0.5 mL 0.2 mg

PC7 APC

APC-A700 APC-A750

UNLB

Freeze-dried

-

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 51 Outside US Flow Cytometry Catalog