Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD25 antigen (IL-2R α chain, Tac or p55) is a single-chain glycoprotein with a molecular weight of 55 kDa which is the low affinity receptor for IL-2 (IL-2R α or IL-2R1). In conjunction with CD122 (IL-2R β , p75) and CD132 (IL-2R γ , p64), CD25 antigen forms the high-affinity IL-2R complex. CD25 is highly expressed on regulatory CD4-positive T lymphocytes and undetected on resting CD8 positive lymphocytes. However, all activated T lymphocytes express the CD25 protein. CD25 is also found on a subset of B cells (CD20 positive), on activated monocytes and macrophages, and on T cell clones. It is also expressed on NK cells enriched from peripheral blood lymphocytes by culturing in the presence of IL-2. It is absent from thymocytes, granulocytes, monocytes, resting NK cells, platelets and erythrocytes. CD25

Lysed normal whole blood sample.

Form Clone: 1HT44H3 (IL-2R1)

Isotype: IgG2a Mouse

Size

Format

Part#

Status

Line Quality Standard

RD1

6604422

0.5 mL

Liquid

ASR

CYTO - STAT

GMP

Clone: B1.49.9

Isotype: IgG2a Mouse

The B1.49.9 monoclonal antibody does not block the binding of IL-2 to its receptor.

Form

Size

Format

Part#

Status

Line Quality Standard

PB

A82944 IM0478U A07774 IM0479U 6607112 IM2646 IM2646U A79386 B92458 A52882 B09684 A86356 B92454

0.5 mL

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid

ASR ASR

IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest

GMP GMP

FITC

2 mL

PE PE

100 tests

CE

98/79/EC

2 mL

ASR RUO

GMP GMP

ECD PC5 PC5

100 tests 100 tests

CE

98/79/EC

1 mL

ASR ASR

GMP GMP

PC5.5 PC5.5

0.5 mL 50 tests

CE

98/79/EC

PC7 APC

1 mL

ASR ASR ASR

GMP GMP GMP

0.5 mL 0.5 mL 50 tests 0.5 mL

APC-A700 APC-A700 APC-A750

CE

98/79/EC

B13979

ASR

GMP

The CD26 antigen is the dipeptidylpeptidase IV ectoenzyme (DPPIV). It is a single-chain glycoprotein with a molecular weight of 110 kDa. It binds to adenosine deaminase protein (ADA). CD26 is a functional collagen receptor. CD26 provides an alternative activation pathway for T lymphocytes in which the CD3 ζ (TCR ζ chain) is required for CD26-mediated signaling. The expression of CD26 is found on resting T cells and is increased on activated T lymphocytes, B lymphocytes, macrophages and NK cells. In a study using this antibody, it has been suggested that CD26 is involved as a cofactor of CD4 in the mechanism of triggering apoptosis. Another study showed the expression of CD26 in human kidney and on lymphocytes and macrophages of specific skin biopsies. CD26

Lysed normal whole blood sample gated on lymphocytes.

Clone: 4EL-1C7 (Ta1)

Isotype: IgG1 Mouse

The 4EL-1C7 monoclonal antibody does not inhibit T cell activation and cytotoxicity.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2724

2 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 50 Outside US Flow Cytometry Catalog