Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD21 molecule is a 145 kDa type I transmembrane glycoprotein, which belongs to the family of complement regulatory proteins. CD21 is expressed by mature B lymphocytes and is rapidly lost following cellular activation. It is not expressed on early (pre-B) and late (B blast or plasma cell) B ontogeny. On follicular dendritic cells the CD21 antigen is strongly expressed. Expression of CD21 in a subset of T lymphocytes has been reported. Four types of CD21 ligands have been identified: They are products following proteolytic cleavage of the complement protein C3 (i. e. iC3b, C3dg and C3d), the Epstein-Barr (EBV) virus envelope, Interferon α (IFN α ) and CD23. CD21 was found to be part of a large complex containing also CD19, and CD81 (also named TAPA-1) suggesting a key-role of CD21 during the immune response. CD21

Lysed normal whole blood sample

Form Clone: BL13

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PB

B09982 IM0473U A32536

0.5 mL

Liquid Liquid Liquid

ASR ASR ASR

IOTest IOTest IOTest

GMP GMP GMP

FITC

2 mL 2 mL

PE

The CD22 antigen (BL-CAM) is a transmembrane glycoprotein of the immunoglobulin superfamily. It may be expressed as two different isoforms: the α form (130 kDa) and the β form (140 kDa). It is a B lineage restricted antigen that occurs in the cytoplasm of early B cell precursors and pre-B cells and on the cell surface of resting and activated B cells. The CD22 antigen is absent from peripheral blood T lymphocytes, granulocytes, monocytes. It is expressed on less than 1% of bone marrow mononuclear cell and 50% of Epstein Barr virus transformed lymphoblastoid B cell lines. CD22 demonstrates heterogeneous expression on resting peripheral blood B lymphocytes. The antigen is lost following activation of B cells with a variety of mitogens. The CD22 molecule regulates signal transduction through the B cell receptor. It is a receptor for certain sialic acid containing glycoproteins. CD22

Lysed normal whole blood sample

Clone: HD239 (B3) Isotype: IgG2b Mouse The B3 antibody binds to an epitope located within the first Ig-like domain of CD22 antigen. It can be used to detect intracytoplasmic CD22 in flow cytometry after permeabilization.

Form

Size

Format

Part#

Status

Line Quality Standard

RD1

6604428

0.5 mL

Liquid

ASR

CYTO - STAT

GMP

Form Clone: SJ10.1H11

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PB

B06498 IM0779U

0.5 mL

Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid

ASR ASR

IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest IOTest

GMP GMP

FITC

2 mL

PE PE

IM1835

100 tests

CE

98/79/EC

IM1835U B10245 IM3704 A80712 A74788 A60791 B96777 A89311 B36293

2 mL

ASR ASR ASR ASR ASR ASR

GMP GMP GMP GMP GMP GMP

ECD PC5

0.5 mL

1 mL

PC5.5

0.5 mL

PC7 APC APC

1 mL

0.5 mL 50 tests 0.5 mL 50 tests

CE

98/79/EC

APC-A700 APC-A700

ASR

GMP

CE

98/79/EC

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 48 Outside US Flow Cytometry Catalog