Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

Fc- γ RIII (CD16) is a low affinity receptor involved in interactions with immune complexes. It is a 50 – 80 kDa protein with two distinct forms encoded by two different genes: IIIA and IIIB. The transmembrane form (Fc- γ RIIIA) is expressed on NK cells, monocytes and macrophages. The IIIB mRNA encodes Fc γ RIIIB, a glycoprotein anchored to the outer leaflet of the membrane by a glycosylphosphatidyl-inositol (GPI) moiety and is transcribed only in human neutrophils. Fc γ RIIIB (CD16b) may act with Fc γ RIIA (CD32) expressed on neutrophils to mediate antibody- dependent cytotoxicity (ADCC) and phagocytocis. The GPI-linked receptor exists in a soluble form (sCD16) revealing an immunoregulatory function through interaction with complement receptors. CD16b

Lysed normal whole blood sample

Clone: 1D3 Isotype: IgM Mouse The monoclonal antibody 1D3 was shown to be non-reactive with NK cells or monocytes but to be specific to polymorphonuclear leucocytes (PMNs). It recognizes an epitope specific to the Fc γ RIIIB molecule and was characterized as the sole antibody belonging to the CD16b cluster of differentiation. This antibody reacts with PMNs, irrespective of the neutrophil antigen (NA) phenotype, although it shows lower reactivity with NA2-homozygote PMNs as compared to NA1- homozygotes or NA1/NA2 heterozygotes.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM2353U

2 mL

Liquid

ASR

IOTest

GMP

CD18 The beta2 integrins (CD11/CD18) are the major adhesion molecule family of leukocytes. Most leukocytes express one or more members of this family. CD18 is the 95 kDa integrin β 2-chain which forms non-covalently-bound heterodimers with one of four α subunits. The four alpha subunits are: CD11a ( α L) (all leukocytes), CD11b ( α M) (granulocytes, monocytes, some macrophages), CD11c ( α X) (granulocytes, monocytes, dendritic antigen presenting cells), CD11d ( α D) (CD8 T-cell subpopulation including large granular lymphocytes, macrophages and gamma-delta T cells in splenic red pulp). CD18 is expressed by all leukocytes (including histiocytes - macrophages, Langerhan's cells and dendritic cells) but no platelets. Macrophages and granulocytes express 10-fold more CD18 than do lymphocytes.

Clone: 7E4

Isotype: IgG1 Mouse

7E4 is a blocking monoclonal antibody in cell adhesion assays.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1568U IM1570U

2 mL 2 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

PE

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 45 Outside US Flow Cytometry Catalog