Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

Beckman Coulter offers the largest portfolio of CE-IVD and ASR conjugated antibodies validated against clinical standards. We develop and manufacture reagents according to current Good Manufacturing Practices (cGMP), the highest quality standards in the industry, ensuring optimal antibody panel performance. We offer a wide variety of antibodies for surface or intracellular staining, with more than 1,000 products available. Our antibody specificities are available in an extensive variety of clones, selected in collaboration with leading research institutions according performance and current cellular analysis areas such as hemato-oncology, immune monitoring or stem cell studies. Our comprehensive conjugated antibody portfolio is designed with proprietary, state-of-the-art chemistry for reduced background and maximum signal. Our proven tandem-dye design ensures stability, minimal spillover, and reproducible results over time. Beckman Coulter’s quality control processes and manufacturing capabilities enable long-term lot-to-lot consistency. • Largest portfolio of conjugated antibodies validated against the clinical standards CE-IVD and ASR. • Beckman Coulter’s tandem dyes stability and lot-to-lot consistency ensure reproducible and reliable results. • Broad portfolio of clones and dyes allows for flexible panel design. Technical applications and cross reactivities of clones are described in the clone index. See page 307-311 The APO2.7 antigen (also named 7A6 antigen) is a 38 kDa protein localized on the membrane of mitochondria whose expression appears to be restricted to cells undergoing apoptosis. The APO2.7 antigen can be detected after apoptosis induction via CD95/Fas ligation, irradiation or drug treatment. Expression of APO2.7 appears as an early event in the apoptosis process. Normal viable cells are negative or weakly positive for APO2.7. Research studies have shown that less than 2% of peripheral T cells from normal donors expressed the APO2.7 antigen. Some level of APO2.7 expression associated with an on-going apoptotic process has been demonstrated in activated T cells. APO2.7 ( 7A6 )

Apoptotic Jurkat cell line

Clone: 2.7A6A3 (APO2.7) Isotype: IgG1 Mouse The APO2.7 antibody can be used for the direct quantitation of apoptotic cells by flow cytometry, after permeabilization with digitonin. Use of APO2.7 allows for precise monitoring of the early and late cellular responses during apoptosis.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2088U

2 mL

Liquid

ASR

IOTest

GMP

The β 2-microglobulin is the non-polymorphic non-covalently bound light chain (12 kDa) of the class I major histocompatibility complex (MHC-I) constitutively expressed on the surface of almost all nucleated cell. β 2-microglobulin structure, stabilized by a disulfide bound, is similar to an immunoglobulin like domain. β 2-microglobulin is required in the endoplasmic reticulum (ER) to associate with the MHC class I heavy molecule to form with other proteins (calreticulin, tapasin, etc.) the loading complex that exits the ER and is transported to the cell surface. After peptide presentation, β 2-microglobulin is actively released into circulation. β 2-microglobulin

Lysed normal whole blood sample

Form Isotype: IgG2a Mouse The B1G6 monoclonal antibody is specific for human β 2-microglobulin. It does not react with β 2-microglobulin from other species. It recognizes the β 2-microglobulin, free or associated with human HLA class I molecules. Part# Format Size Status Line Quality Standard UNLB - RUO IM0114 Freeze-dried 0.2 mg GMP Clone: B1G6

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 29 Outside US Flow Cytometry Catalog