Flow Cytometry catalog - ROW 2019
DURAClone Panels
Size
Status
Part #
DURAClone B27 Reagent Kit
50 tests
CE
B36862
The DURAClone B27 Reagent Kit comprises a two-color, immunofluorescence stain, suitable for detecting the HLA-B27 antigen expression in erythrocyte-lysed, human whole blood samples (for example, a lyse and no wash protocol), using a flow cytometer. This reagent is not intended to be used to determine the HLA-B27 tissue group.
The Human Leukocyte Antigen (HLA) B27 is a Class I surface antigen encoded by the B locus in the major histocompatibility complex (MHC) that presents microbial antigens to T-lymphocytes. The onset of seronegative spondyloarthropathies that include ankylosing spondylitis, Reiter's disease, psoriatic arthritis and inflammatory bowel disease are associated with the expression of HLA-B27 antigen on T-lymphocytes. Screening for HLA-B27 is thus of clinical relevance in conjunction with the symptomatic presentation of the disorder. Micro-lymphotoxicity tests are conventionally used in HLA-typing but are time-consuming and expensive. Flow cytometry has gradually evolved into a faster and reliable method for screening samples for HLA-B27 antigen. T-lymphocytes selected through gating of the CD3 + specific population are analyzed for staining by HLA-B27 conjugates. It has been demonstrated that this pre- selection of T-lymphocytes increases the specificity of the test by eliminating the background attributed to other leukocyte populations. Another method of lowering the risk of false-positives is by the addition of an Anti-B7 antibody to the reagent cocktail. The Anti-B7 antibody competes with the Anti-B27 antibody for the B7 antigen and in this manner, suppressing the incidence of cross- reactivity of Anti-B27 antibodies to B7 antigens. The DURAClone B27 Reagent Kit contains two clones of the Anti-HLA-B27 antibody and an Anti -B7 antibody. The presence of these antibodies improves the specificity of the reagent. The Anti-B7 antibody binds preferentially to the B7 antigen, thus reducing cross-reactivity. The test result is a direct extrapolation of the fluorescent staining intensity of the HLA-B27 conjugates with respect to a cutoff value. A protocol, specific to BD Biosciences* instruments, is detailed in the B27 Reagent Kit IFU (Instruction for Use).
Components
Specificity
Fluorochrome
Clone (Isotype)
Size
CD3-PE-Dy649/HLA-B27-PE/HLA-B7
50 tubes
CD3
PE-Dy649
UCHT1 (IgG1 Mouse)
HLA-B27
PE
HLA-ABC-m3 (IgG2a Mouse)
HLA-B27
PE
FD705 (IgG2b Mouse)
HLA-B7
-
BB7.1 (IgG1 Mouse)
DuraLyse Solution
1 x 25 mL
-
-
Not available in all geographies. DURAClone Dry Reagents are available for IVD use only in those markets for which they have been registered per applicable regulations.
ANTIBODIES & KITS
186
Outside US Flow Cytometry Catalog
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