Flow Cytometry catalog - ROW 2019

DURActive Panels

Functional assays such as induction of cytokine expression typically involve time-critical thawing of frozen stimulation cocktails and liquid handling of resulting small volumes. The DURActive stimulation kits eliminate freeze-storage and error-prone liquid handling as they are dry ready-to-use stimulation mixes in unitized 1-test-format, stable at room temperature. The DURActive dry stimulation kits ensure reliability of the first crucial step in cellular activation assays before a precisely coordinated signaling cascade can yield its response.

The DURActive stimulation kits contain optimally dosed physiologically active compounds provided as unitized single tests in 12x75 mm tube format.

• Unitized dry Beckman Coulter proprietary format avoids freezing, thawing, diluting and pipetting • Pre-formulated mixes containing 2-3 active compounds at expert-proven optimal dose • Safe handling of dry stimulation mixes due to sub-hazardous amounts of stimulating agents without organic solvents • Convenient 25 tests / package

Size

RUO Status

Part #

DURActive 1

C11101

25 tests

Dry Stimulation Mixes for Immune Functional Assays Phorbol-Myristate-acetate (PMA) and Ionomycin are broad cellular activators of human leukocytes. Brefeldin A blocks Golgi-apparatus-mediated secretion. A mix of these 3 compounds strongly activates leukocytes and traps newly synthesized biomolecules (e.g. cytokines) inside the cell, hence it can serve e.g. as positive control for leukocyte (ligand-specific) functional assays and as a nonspecific activator in leukocyte functional subtyping experiments.

The DURActive stimulation kits contain optimally dosed physiologically active compounds provided as unitized single tests in 12x75 mm tube format.

Components

Phorbol 12-myristate 13 acetate (PMA)

Ionomycin calcium salt from Streptomyces conglobatus

Brefeldin A

Not available in all geographies. DURActive products are available for Research Use Only. Not for use in diagnostics procedures.

ANTIBODIES & KITS

183

Outside US Flow Cytometry Catalog