Flow Cytometry catalog - ROW 2019

DURAClone

DURAClone RE

The dry DURAClone RE panels enable sensitive detection of rare abnormal cell populations in blood or bone marrow using carefully optimized and highly dosed antibody combinations. DURAClone tubes contain a coating of dry ready-to-use antibody panel stable at room temperature (between 20 and 30°C, in a dry place) for worry-free storage and shipping. The complete elimination of antibody pipetting minimizes human error sources and purges the workflow from repetitive actions that are expensive to automate. Reagent inventory management and cumbersome lot-to-lot equivalence testing are reduced to a minimum. Three dried reagent single color compensation kits are provided with each package of 25 assay tubes. • Solutions for rare event cytometry. • Designed and optimized together with key experts. • Standardized for use in clinical research studies.

Size

Status

Part #

DURAClone RE CLB Tube

B80393

25 tests

RUO

Detection of CD45 + CD19 + ROR1 + CD5 ++ CD43 ++ CD81 - CD20 - CD79b - B cells.

Mature human peripheral B cells (CD45+CD19+ lymphocytes) express high density levels of the antigens CD20, CD79b and CD81 on their surface. In contrast, the antigens CD5, CD43 and ROR1 are expressed at low density levels limited to a small fraction of these cells. Deviation from these patterns involving high expression of CD5, CD43 and ROR1 accompanied by reduced expression densities of CD20, CD79b, and CD81 can be utilized to identify abnormal B cells even at very low frequencies.

The DURAClone RE CLB tube is an 8-color, 8-monoclonal antibody reagent that allows the identification of abnormal B cells in human whole blood samples by eight color flow cytometry. It contains 3 Compensation Kits, each kit containing eight tubes, each of a single color: CD4-FITC; CD4-PE; CD79b-PC5.5; CD19-PC7; CD4-APC; CD43- APC-A750; CD4-PB; CD8-Krome Orange. All tandem dye-labeled antibodies are lot-matched.

Specificity

Fluorochrome

Clone (Isotype)

CD20

Pacific Blue*

B9E9 (IgG2a Mouse)

CD45

Krome Orange

J33 (IgG1 Mouse)

CD81

FITC

JS64 (IgG2a Mouse)

ROR1

PE

2A2 (IgG1 Mouse)

CD79b

PC5.5

CB3-1 (IgG1 Mouse)

CD19

PC7

J3-119 (IgG1 Mouse)

CD5

APC

CLB-T1/1 (IgG1 Mouse)

CD43

APC-Alexa Fluor* 750

DFT1 (IgG1 Mouse)

Not available in all geographies. DURAClone IF, IM, RE and SC Panels are available for Research Use Only. Not for use in diagnostics procedures.

ANTIBODIES & KITS

179

Outside US Flow Cytometry Catalog