Flow Cytometry catalog - ROW 2019

DURAClone

Size

Status

Part #

DURAClone IF Basophil Activation Tube

C23406

25 tests

RUO

Characterization of the activation status of basophils. Basophils play a key role in immediate hypersentivity as primary effector cells. These cells express the high affinity receptor for IgE (Fc ε R1). Allergens induce basophils activation by cross-linking surface IgE leading to release of mediators and expression of activation markers on the basophils surface such as CD63 and CD203c. The analysis of level of expression of CD63 and CD203c antigen allows the characterization of resting and activated basophils in an IgE-dependent response to allergens in sensitized individuals. Interestingly, CD203c and CD63 expression do not always correlate as they seem to implicate 2 different pathways of basophils activation where CD63 will reflect anaphylactic degranulation and CD203c piecemeal degranulation

The DURAClone IF Basophil Activation tube kit consists of 25 tubes of a dried optimized five-color combination of fluorescent monoclonal antibodies (CD203c-PE, CD3-PC7, CD63-PBE, CD45-KrO, CRTH2-AF647) as well as 5 tubes of a positive control containing the same dried five color cocktail along to an anti-IgE also dried.

Specificity

Fluorochrome

Clone (Isotype)

CD203c

PE

97A6 (IgG1 Mouse)

CD3

PC7

UCHT1 (IgG1 Mouse)

CD294

Alexa Fluor* 647

BM16 (IgG2a Rat)

CD63

Pacific Blue*

CLB-Gran/12 (IgG1 Mouse)

CD45

Krome Orange

J33 (IgG1 Mouse)

Size

Status

Part #

Ca2+ Activation solution

C23407

100 tests

RUO

The Activation Solution is a calcium enriched buffer that restores physiological concentration of Ca2+ in EDTA anti coagulated blood, thus allowing to conduct Ca2+ dependent functional studies.

EDTA (ethylene-diamine-tetraacetic acid) is a broadly used Ca2+-depleting anti-coagulant for whole blood samples that interferes with functional assays assessing/characterizing Ca2+-dependent cellular functions. Treatment of EDTA-anti-coagulated blood with the Ca2+ Activation Solution can enable certain Ca2+ dependent functional studies. The feasibility of the assay with the Ca2+ Activation solution should be evaluated for each experimental set-up.

Specificity

Fluorochrome

Clone (Isotype)

Not available in all geographies. DURAClone IF, IM, RE and SC Panels are available for Research Use Only. Not for use in diagnostics procedures.

ANTIBODIES & KITS

177

Outside US Flow Cytometry Catalog