Flow Cytometry catalog - ROW 2019

DURAClone

DURAClone IF

The DURAClone IF panels reveal immune function utilizing a streamlined and simplified intracellular staining workflow. The sensitive and specific characterization of a cytokine response at the single cell level demonstrates the unique strength of cytometry. When using dry pre-formulated DURAClone IF antibody panels you can be certain that the detection of cellular responses is not biased by antibody pipetting variability or suboptimal panel design. Each DURAClone IF kit contains 25 assay tubes together with 3 single color compensation kits in case compensation is required for the instrument setup. • Pre-formulated 4-6 color antibody panels • Unitized dry Beckman Coulter proprietary format • Expert-proven marker combinations • Open slots for additional drop-in markers • Designed for minimal spillover into detectors for functional read-out parameters • Standardized for use in clinical research studies

Size

Status

Part #

DURAClone IF T Activation Tube

B88649

25 tests

RUO

Detection of proinflammatory cytokines in T cells T cells represent a central subset of the adaptive immune system. Upon recognition of specific antigens T cells - among other responses – secrete inflammatory cytokines such as IFN- γ , TNF- α and IL-2 which stimulate complementary parts of the immune system to defend the host against the provoking pathogens. Identification of cytokine-secreting T cells subsets is an important tool in studying and understanding T cell function in immune activation and suppression.

The DURAClone IT T Activation tube is a 6-color reagent that allows the identification of IFN γ , TNF α and IL2 secreting CD4 + and CD8 + T cells. It contains 3 compensation Kits, each kit containing six tubes, each of a single color: CD4-FITC; CD4-PE; IL2-PC7; CD8-AF700; CD3-AF750; CD4-PB. All tandem dye-labeled antibodies are lot-matched.

Specificity

Fluorochrome

Clone (Isotype)

CD4

Pacific Blue*

13B8.2 (IgG1 Mouse)

IFN- γ

FITC

45.15 (IgG1 Mouse)

TNF α

PE

IPM2 (IgG1 Mouse)

IL-2

PC7

MQ1-17H12 (IgG2a Rat)

CD8

Alexa Fluor* 700

B9.11 (IgG1 Mouse)

CD3

Alexa Fluor* 750

UCHT1 (IgG1 Mouse)

Not available in all geographies. DURAClone IF, IM, RE and SC Panels are available for Research Use Only. Not for use in diagnostics procedures.

ANTIBODIES & KITS

175

Outside US Flow Cytometry Catalog