Flow Cytometry catalog - ROW 2019

DURAClone

Size

Status

Part #

DURAClone IM Count

C00162

25 tests

RUO

Although flow cytometry only provides relative quantitation of cell number, it is possible to determine the absolute cell number using reference beads of known concentration mixed with the human whole blood sample of interest.

This test is based on the ability of specific monoclonal antibodies to bind to the antigenic determinants expressed by lineage committed cells and the binding of membrane impermeant 7-aminoactinomycin D to the DNA of dead cells due to their lost membrane integrity. Additionally, the mixing of a known number of beads with a known volume of a whole blood can be used to determine the number of cells in the whole blood per unit volume.

The DURAClone IM Count is used for enumeration of CD45+ cells in whole blood while simultaneously differentiating live and dead cells.

Specificity

Fluorochrome

Clone (Isotype)

CD45

FITC

J33 (IgG1 Mouse)

7-AAD

-

-

Fluorescent Beads

-

-

Not available in all geographies. DURAClone IF, IM, RE and SC Panels are available for Research Use Only. Not for use in diagnostics procedures.

ANTIBODIES & KITS

174

Outside US Flow Cytometry Catalog