Flow Cytometry catalog - ROW 2019

DURAClone

Size

Status

Part #

DURAClone IM Treg Tube

B53346

25 tests

RUO

Regulatory T cells (Tregs) play a crucial role in the induction and maintenance of immunological tolerance. Together with CD4 and CD25, the expression of the transcription factor FoxP3 is considered the hallmark of human regulatory T cells (CD3+ CD4+ CD25+ FoxP3+). Helios, a transcription factor of the Ikaros family, has been described as a marker identifying thymus-derived nTregs - as opposed to Tregs induced in peripheral tissues (iTregs). Additionally, Helios acts as an enhancer of regulatory function. Surface expression of ectoenzyme CD39 exerts a suppressive function by its ATPase activity and is restricted to an effector/memory-like subpopulation of Tregs. CD45RA surface expression indicates a naïve or unprimed subpopulation of Tregs.

The DURAClone IM Treg Tube is an 8-color, 8-monoclonal antibody reagent that allows the identification of subpopulations of regulatory T cells in human whole blood samples by eight color flow cytometry. It contains 3 Compensation Kits, each kit containing eight tubes, each of a single color: CD4-FITC; CD4-PE; CD39-PC5.5; CD4-PC7; FoxP3-A647; CD3-APC-A750; CD4- Pacific Blue; CD8-Krome Orange. All tandem dye-labeled antibodies are lot- matched.

Specificity

Fluorochrome

Clone (Isotype)

CD45RA

FITC

2H4LDH11LDB9 (IgG1 Mouse)

CD25

PE

B1.49.9 (IgG2a Mouse)

CD39

PC5.5

BA54 (IgG1 Mouse)

CD4

PC7

SFCI12T4D11 (IgG1 Mouse)

FoxP3

Alexa Fluor* 647

259D (IgG1 Mouse)

CD3

APC-Alexa Fluor* 750

UCHT1 (IgG1 Mouse)

Helios

Pacific Blue*

22F6 (IgG Hamster)

CD45

Krome Orange

J33 (IgG1 Mouse)

Not available in all geographies. DURAClone IF, IM, RE and SC Panels are available for Research Use Only. Not for use in diagnostics procedures.

ANTIBODIES & KITS

172

Outside US Flow Cytometry Catalog