Flow Cytometry catalog - ROW 2019

DURAClone

Size

Status

Part #

DURAClone IM TCRs Tube

B53340

25 tests

RUO

Mature T cells - as identified by positive surface CD3 staining - express T cell receptors (TCRs) that enable low affinity-recognition of antigens bound to major histocompatibility complex (MHC) molecules. MHC co-binding of either CD4 (MHC class II) or CD8 (MHC class I) enhances and prolongs the engagement with the antigen-presenting cells. Two forms of TCRs are known either consisting of heterodimers of variable α and β protein chains or γ and δ protein chains. Proportions of 95% αβ T cells and 5% γδ T cells are commonly found in blood .The largest subset of γδ T cells can be identified by the IMMU 389 antibody clone recognizing the γδ complex from the V δ 2+ T cell receptor (TCR γδ V δ 2+), being mostly associated with V γ 9. A smaller subset of γδ T cells is staining positive with the R9.12 antibody clone that binds to the V δ 1 segment of γδ T cells (TCR γδ V δ 1+). The relative sizes of these two subsets have been assigned a role in feto-maternal and allograft tolerance.

The DURAClone IM TCRs Tube is a 9-color, 9-monoclonal antibody reagent that allows the identification of the TCR subtypes on the surface of mature T cells.

It contains 3 Compensation Kits, each kit containing 9 tubes, each of a single color: CD4-FITC, CD4-PE, HLA DR-ECD, V δ 1-PC7, CD4-APC, CD8-A700, CD3-APC-A750, CD4-Pacific Blue, CD8-Krome Orange. All tandem dye-labeled antibodies are lot-matched.

Specificity

Fluorochrome

Clone (Isotype)

TCR γδ

FITC

IMMU510 (IgG1 Mouse)

TCR αβ

PE

IP26A (IgG1 Mouse)

HLA-DR

ECD

Immu-357 (IgG1 Mouse)

TCRV δ 1

PC7

R9.12 (IgG1 Mouse)

CD4

APC

13B8.2 (IgG1 Mouse)

CD8

Alexa Fluor* 700

B9.11 (IgG1 Mouse)

CD3

APC-Alexa Fluor* 750

UCHT1 (IgG1 Mouse)

TCRV δ 2

Pacific Blue*

IMMU 389 (IgG1 Mouse)

CD45

Krome Orange

J33 (IgG1 Mouse)

Not available in all geographies. DURAClone IF, IM, RE and SC Panels are available for Research Use Only. Not for use in diagnostics procedures.

ANTIBODIES & KITS

171

Outside US Flow Cytometry Catalog