Flow Cytometry catalog - ROW 2019

DURAClone

Size

Status

Part #

DURAClone IM Dendritic cell Tube

B53351

25 tests

RUO

In human blood, DC can be separated in two main subsets, namely the myeloid DCs (mDCs) and the plasmacytoid DCs (pDCs). Both subsets share the lack of expression of classical mature lymphocytes lineage markers, CD3, CD19, CD20 and CD56 as well as the monocytic CD14 and a high expression level of HLA-DR. mDCs and pDCs differ by their CD11c expression, with mDCs expressing high level of CD11c while pDCs do not express CD11c. pDCs, which are distinguished by the expression of CD123, are less potent to present antigen but constitute the 1st barrier in anti-viral immunity. Upon virus exposure, pDCs produce large amount of Type I interferons. mDCs are highly efficient in antigen presentation. In blood, 3 subsets of mDCs are identified: CD1c+ mDCs (MDC1), CD141+ mDCs (MDC2), and CD16+ mDCs.MDC1 have been described as key players in the induction of immunity towards extracellular pathogens. MDC2 are suggested to drive the CD8+ T cell-mediated immune response. Recently, it has been shown that MDC2 express CLEC9A, a C-type lectin domain family. As opposed to CD141, CLEC9A presents the advantage of being expressed in both human and mouse DCs. Thus, it can serve as a DC marker crossing species barriers and may help in the translation from the mouse DC system to human immunobiology. CD16+ mDCs have been described as inflammatory dendritic cells.

The DURAClone IM Dendritic cells Tube is an 8-color, 12-monoclonal antibody reagent that allows the identification of common extracellular markers of different DC Subsets, present in whole blood specimens. It contains 3 Compensation Kits, each kit containing eight tubes, each of a single color: CD4-FITC; CD4-PE; CD1c-PC5.5; CD11c-PC7; CD4-APC; CD123-APCA700; CD4- Pacific Blue; CD8-Krome Orange. All tandem dye-labeled antibodies are lot- matched.

Specificity

Fluorochrome

Clone (Isotype)

CD16

FITC

3G8 (IgG1 Mouse)

Lineage (CD3/CD14/ CD19/CD20/CD56)

UCHT1 (IgG1 mouse)/RMO52 (IgG2a mouse)/J3-119 (IgG1 mouse)/ B9E9 (IgG2a mouse)/N901 (IgG1 mouse)

PE

CD1c

PC5.5

L161 (IgG1 Mouse)

CD11c

PC7

BU15 (IgG1 Mouse)

Lysed normal whole blood sample

Clec9A

APC

8F9 (IgG2a Mouse)

CD123

APC-Alexa Fluor* 700

SSDCLY107D2 ( IgG1 Mouse)

HLA-DR

Pacific Blue*

Immu-357 (IgG1 Mouse)

CD45

Krome Orange

J33 (IgG1 Mouse)

Not available in all geographies. DURAClone IF, IM, RE and SC Panels are available for Research Use Only. Not for use in diagnostics procedures.

ANTIBODIES & KITS

170

Outside US Flow Cytometry Catalog