Flow Cytometry catalog - ROW 2019

DURAClone

Size

Status

Part #

DURAClone IM T cell subsets Tube

B53328

25 tests

RUO

When studying the immune response, T cell-mediated immunity is the central component. After their maturation in the thymus, T cells circulate in blood and in the lymphatic system as naive T cells expressing lymph node homing antigens such as CD197/ CCR7. Upon exposure to foreign antigens through encounter with specialized, antigen-presenting cells in secondary lymphoid tissues, T cells become antigen-specific effector cells associated with loss of CD27 and CD28 expression. Along with the effector cells, long-living central memory (CD45RA-CCR7+) and effector memory T cells (CD45RACCR7-) are generated to achieve and preserve the ability of rapid antigen-specific immune response. Terminal effector stages of T cell differentiation are indicated by upregulation of CD57 (effector phenotype) and CD279/PD-1 (co-inhibitory molecule, exhausted phenotype) expression.

The IM T cell subsets Tube is a 10-color, 10-monoclonal antibody reagent that allows the identification of common extracellular markers of different cell subsets, present in whole blood specimens. It contains 3 Compensation Kits, each kit containing ten tubes, each of a single color: CD4-FITC, CD4-PE, CD28-ECD, CD279-PC5.5, CD27-PC7, CD4-APC, CD8-A700, CD3-APC-A750, CD4-Pacific Blue, CD8-Krome Orange. All tandem dye-labeled antibodies are lot-matched.

Specificity

Fluorochrome

Clone (Isotype)

CD45RA

FITC

2H4LDH11LDB9 (IgG1 Mouse)

CD197

PE

G043H7 (IgG2a Mouse)

CD28

ECD

CD28.2 (IgG1 Mouse)

CD279

PC5.5

PD1.3 (IgG2b Mouse)

CD27

PC7

1A4CD27 (IgG1 Mouse)

CD4

APC

13B8.2 (IgG1 Mouse)

CD8

Alexa Fluor* 700

B9.11 (IgG1 Mouse)

CD3

APC-Alexa Fluor* 750

UCHT1 (IgG1 Mouse)

CD57

Pacific Blue*

NC1 (IgM Mouse)

CD45

Krome Orange

J33 (IgG1 Mouse)

Not available in all geographies. DURAClone IF, IM, RE and SC Panels are available for Research Use Only. Not for use in diagnostics procedures.

ANTIBODIES & KITS

169

Outside US Flow Cytometry Catalog