Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

Tumor necrosis factor α (TNF α ) or cachectin is a cytokine that was first identified based on its cytotoxic activity against various cell lines and tumor cells. Human TNF α is synthesized as a pro-protein with a molecular weight of 26 kDa (membrane-bound form). The pro-protein is cleaved by a specific metalloprotease (TACE) yielding a monomeric, soluble form of 17 kDa. Under physiological conditions, TNF α forms a homotrimeric protein. This cytokine is primarily produced by mononuclear phagocytes, and by activated lymphocytes. Immunostaining allows intracellular detection of TNF α before it can be detected by a bioassay. TNF α acts on target cells by binding to two types of receptors, the TNF α receptor I (TNF-RI or CD120a) and the TNF α receptor II (TNF-RII or CD120b). TNF α elicits a wide spectrum of immune and inflammatory responses, including the induction of other cytokines and immunoregulatory molecules, cell growth and differentiation and apoptosis. This molecule is also involved in Th1 - Th2 (T helper 1 - T helper 2) cytokine pathways (or Type 1 - Type 2 responses), as a Th1- effector cytokine. TNF α

Activated PBL.

Form Clone: IPM2 (188)

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

IM3279U B76295

2 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

AF700

0.5 mL

Zap-70, a Syk family protein tyrosine kinase expressed in T and NK cells, plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: autophosphorylation and transphosphorylation by the Src family tyrosine kinase, Lck. Tyrosine phosphorylation of Zap-70 correlates with its increased kinase activity and downstream signaling events. ZAP-70

Lysed normal whole blood sample.

Clone: SBZAP Isotype: IgG1 Mouse The monoclonal antibody SBZAP was generated against a KLH-peptide sequence corresponding to the human ZAP-70 amino acid residues 280-309.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

B57658 B10739

50 tests 50 tests

Liquid Liquid

RUO RUO

IOTest

GMP GMP

UNLB

-

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 144 Outside US Flow Cytometry Catalog