Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

Thrombin is a coagulation protease produced at sites of vascular injury that activates platelets, endothelial cells, leukocytes and mesenchymal cells via cleavage of specific cell surface receptors known as proteinase activated receptors (PARs). A functional thrombin receptor from human platelets has been cloned and sequenced. It is a 66-kDa, single polypeptide chain that belongs to the cell surface G-protein-coupled receptor family, with seven transmembrane domains and an extracellular N terminus. The thrombin cleavage site is located in the N terminus between Arg41 and Ser42. Following cleavage by thrombin, activated receptors undergo desensitization and internalization but a fraction of them are recycled to the cell surface. The Thrombin Receptor is expressed on a variety of cells including platelets and endothelium. The receptor is a substrate of thrombin and has been shown to play a role in the activation of platelets. Thrombin receptor Isotype: IgG1 Mouse The SPAN 12 antibody recognizes an epitope on the receptor that spans the thrombin cleavage site. It binds to intact receptors, but not to cleaved ones. SPAN 12 inhibits thrombin-induced receptor activation at reduced thrombin concentrations. Clone: SPAN 12

HEL Cell line.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2583

2 mL

Liquid

ASR

IOTest

GMP

Clone: WEDE15 Isotype: IgG1 Mouse The WEDE15 antibody recognizes an epitope on the receptor, distant from the N-terminal thrombin cleavage site (WEDEE). It reacts with both intact and cleaved receptors.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2584

2 mL

Liquid

ASR

IOTest

GMP

The T cell intracellular antigen 1 (TIA-1) is a 17 kDa cytoplasmic granule-associated protein, expressed in cells possessing cytolytic potential. TIA-1 is also designated as GMP-17 (granule membrane protein of 17 kDa). The GMP-17/TIA-1 molecule could be involved in the molecular signaling cascade during Fas (CD95)-mediated apoptosis. In normal peripheral blood lymphocytes, TIA-1 is expressed by 90% of CD16+, 50-60% of CD8+, and less than 10% of CD4+ cells. Although TIA-1 may be detected in unstimulated T lymphocytes, activated T lymphocytes are induced to express disulfide linked polymers of the 17 kDa protein, also recognized by TIA-1. TIA-1 is expressed by all monocytes and granulocytes, by activated CD4+ T-cell clones and activated natural killer cell clones, and by Con A-activated thymocytes. On the contrary, TIA-1 is not expressed by B lymphocytes or B-cell lines. TIA-1

Lysed normal whole blood sample.

Clone: 2G9A10F5 Isotype: IgG1 Mouse The 2G9 antibody is a useful reagent for detecting cytolytic effector cells in tissue infiltrates. It was evaluated during HLDA 5 in the section of monoclonal antibodies reactive with intracellular antigens. It may be used to detect intracytoplasmic TIA-1 in flow cytometry after permeabilization with IntraPrep permeabilization reagent.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM3293 IM2550

2 mL

Liquid

ASR ASR

IOTest

GMP GMP

UNLB

0.2 mg

Freeze-dried

-

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 143 Outside US Flow Cytometry Catalog