Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The ζ subunit is a 16 kDa surface molecule associated with the T cell receptor. Its expression is restricted to T lymphocytes and NK cells. In T lymphocytes, the ζ subunit is expressed as a disulfide-linked homodimer of 32 kDa or as a disulfide-linked heterodimer with a 21 kDa subunit designated η . Both of these dimeric species are non-covalently associated with the remaining subunits of the CD3-TCR-antigen receptor complex. In NK cells, the ζ subunit is expressed as a disulfide-linked homodimer of 32 kDa or as a disulfide-linked heterodimer with a 12 kDa subunit. It is associated with the CD16 molecule (Fc γ RIII). TCR ζ Form Isotype: IgG1 Mouse The 2H2D9 (TIA-2) antibody recognizes a cytoplasmic epitope of the ζ subunit of the T lymphocyte receptor complex. It is suitable for immunoprecipitation, immunoblotting, and flow cytometric analysis of permeabilized cells. The TIA-2 antibody was evaluated during the Fifth International Workshop on Human Leukocyte Differentiation Antigens. TIA-2 antibody can be used to detect intracytoplasmic TCR- ζ in flow cytometry after permeabilization with digitonin. Part# Format Size Status Line Quality Standard PE IOTest ASR IM3169 Liquid 2 mL GMP Terminal deoxynucleotidyl transferase (TdT) is a nuclear enzyme with a molecular weight of 58 kDa. This molecule catalyses random addition of nucleotides on the 3’ OH termini of single- stranded DNA. TdT differs from other DNA polymerases because it does not require a template sequence for polymerization. TdT is mainly expressed during the maturation of the immune system in primary and secondary lymphoid organ cells: i.e. precursors of B lymphocytes in the bone marrow and precursors of T-lymphocytes in the thymus. The TdT expression is not found in myeloid, erythroid, or megakaryocytic precursors, or in other non lymphoid cell components in every tissue, including mesenchymal, epithelial, and germ cells. TdT Clone: 2H2D9 (TIA-2)

JURKAT Cell line.

MOLT4 Mix Cytotrol Cell line.

Form Clone: HT1 + HT4 + HT8 + HT9 Size

Isotype: IgG Mouse

Format

Part#

Status

Line Quality Standard

FITC FITC

IM3524

50 tests

Liquid Liquid

CE

- -

98/79/EC

IM3524U

1 mL

ASR

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 142 Outside US Flow Cytometry Catalog