Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The TCR is a molecular complexe wich comprises two units: a recognition unit, composed of either alpha-beta or gamma-delta heterodimer, which are present on the cell surface in a mutually exclusive manner, and, a transducing unit, the CD3 complex, common to alpha-beta and gamma-delta heterodimers, which triggers the T cell when the recognition unit is occupied by the antigen. The recognition unit recognizes foreign antigens and the diversity necessary for this function of recognition is generated by somatic recombination the TCR genes. There are four TCR gene loci (alpha, beta, gamma and delta). Each of them is composed of several V (variable) segments, coding for about 90 amino acids, very short D (diversity) segments (alpha and delta loci only), and short J (joining) segments (about 15 amino acids), and one or two C (constant) segments. Most of T cells express the alpha/beta TCR (T cell receptor) protein and a small population of T cells expresses the gamma/delta TCR, which usually has a double negative (CD4/CD8) phenotype. Gamma/delta T-cells are normally the first line of defence at epidermal and epithelial surfaces and they represent 10-12% of lymphocytes in the spleen. TCR PAN γ / δ Isotype: IgG1 Mouse The IMMU510 monoclonal antibody recognizes all γ / δ T cells, regardless of their variable chains. It stains 1.6% to 8.9% of peripheral CD3 + cells from healthy donors. In Western blot, it recognizes the δ chain in reducing conditions. It immunoprecipitates the three isoforms of the γ / δ T cell receptor. This antibody is suitable for immunohistochemistry on frozen sections. It has been used to study the distribution of γ / δ T cells and their functions. Part# Format Size Status Line Quality Standard FITC IOTest ASR IM1571U Liquid 1 mL GMP FITC IOTest CE B49175 Liquid 50 tests 98/79/EC PE IOTest ASR IM1418U Liquid 1 mL GMP PE IOTest CE B49176 Liquid 50 tests 98/79/EC PC5 IOTest CE IM2662 Liquid 50 tests 98/79/EC PC5 IOTest ASR IM2662U Liquid 0.5 mL GMP PC5.5 IOTest ASR A99021 Liquid 0.5 mL GMP PC7 IOTest ASR B10247 Liquid 0.5 mL GMP UNLB - RUO IM1349 Freeze-dried 0.1 mg GMP The iValpha7.2 segment in humans is an evolutionarily conserved invariant TCRalpha chain, expressed in mucosal associated invariant T (MAIT) cells, also called mNKT. MAIT cells are abundant in human blood (1-8% of T cells vs. 0.01-1% for NKT cells), the intestinal mucosa and mesenteric lymph nodes (MLN). MAIT cells are evolutionarily conserved innate-like T cells with anti-microbial properties. They are apparently also involved in non-infectious inflammatory disorders and in autoimmune inflammatory lesions. MAIT cells were found to specifically accumulate in the lamina propria (LP) of the intestine. This suggests that these cells may, in fact, be directed to microbial antigens presented by MR1 molecules, having a role of natural killer T cells (mNKT cells) in intestinal immunology. mNKT cells are a subset of non- conventional T cells recognizing endogenous and / or exogenous glycolipid antigens when presented by the major histocompatibility complex (MHC) class I-like antigen-presenting molecules CD1d and MR1. Upon T-cell receptor (TCR) engagement, mNKT cells can rapidly produce various cytokines that have important roles in mucosal immunity. TCR V α 7.2 Clone: IMMU510 Also called TCRAV7S2 according to the nomenclature from Arden et al.

Lysed normal whole blood sample.

Form

Lysed normal whole blood sample. Gating on lymphocytes. Dual staining with CD3-PB.

Clone: 3C10

Isotype: IgG1 Mouse

Form The monoclonal antibody 3C10 recognizes the Va7.2-Ja33 TCR α chain. Part# Format Size

Status

Line Quality Standard

FITC

B90400

50 tests

Liquid

RUO

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 127 Outside US Flow Cytometry Catalog