Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

OSCAR

OSCAR, an abbreviation of osteoclast-associated receptor, is a new member of the leukocyte receptor complex. Human OSCAR is a cell-surface, monomeric N-glycosylated protein of approximately 45 kDa. The amino acid sequence shows homology with that of members of the KIR family. The protein exhibits two C2-type immunoglobulin extracellular domains, a transmembrane section that contains a positively charged arginine residue, and a short intracytoplasmic tail with no recognizable signaling motif. As suggested by the presence of the charged residue, OSCAR associates with the ITAM-adapter FcR γ and not with other signaling molecules. The gene coding for OSCAR has been mapped to chromosome 19q13. Unlike mouse OSCAR, human OSCAR is widely expressed in cells of the myeloid lineage, and in particular, at all stages of dendritic cell (DC) differentiation and maturation. T cells, B cells, and NK cells do not express OSCAR. OSCAR is involved in antigen presentation and activation of human DCs. It allows processing and presentation of exogenous antigens.

Lysed normal whole blood sample.

Form Clone: 11.1CN5

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

A24987

100 tests

Liquid

RUO

IOTest

GMP

Perforin is a 70 kD N-glycosylated modified cytolytic protein. Following synthesis and post- translational modifications, perforin monomers are packaged into lysosome-like cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Both types of cells kill their cellular targets by mechanisms that require direct contact between the effector and target cells. Cytoplasmic granule toxins, predominantly perforin, and a family of structurally related serine proteases (granzymes) with various substrate specificities are secreted by exocytosis and together induce apoptosis of the target cell. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin

Lysed normal whole blood sample.

Form Clone: dG9

Isotype: IgG2b Mouse

Size

Format

Part#

Status

Line Quality Standard

PB

B46030

0.5 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 125 Outside US Flow Cytometry Catalog