Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

Lactoferrin is a 76 kDa single chain polypeptide with iron-binding property. This protein shows structural similarity to the plasma iron-transport protein transferrin. Lactoferrin is involved in various immuno-regulatory functions associated with anti-infective and inflammatory responses such as antibody synthesis, cytokine production, NK cell cytotoxicity, complement activation and lymphocyte proliferation. Lactoferrin synthesis occurs at the myelocytic stage during polymorphonuclear neutrophil (PMN) ontogeny and constitutes a component of secondary (or specific) granules. Unlike the myeloperoxydase, lactoferrin is absent from primary (or azurophilic) granules and appears later during maturation of the granulocytic lineage. Thus, in peripheral blood, intracellular lactoferrin expression is restricted to neutrophils. Lactoferrin

Lysed normal whole blood sample.

Clone: CLB13.17 Isotype: IgG1 Mouse The PE-conjugated CLB-13.17 antibody is optimized for flow cytometric detection of intracellular lactoferrin by flow cytometry after permeabilization with IntraPrep permeabilization reagent.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2742

2 mL

Liquid

ASR

IOTest

GMP

Myeloperoxidase (MPO) is an heterodimeric glycoprotein of 150 kDa with an α 2/ β 2 structure. The two subunits ( α and β ) have a molecular weight of 55 and 15 kDa, respectively. MPO synthesis occurs in bone marrow at an early stage of myeloid lineage differentiation. MPO is specifically expressed during promyelocytic formation, the stage at which azurophilic granules (or primary granules) are formed. MPO is still found in mature myeloid cells, becoming the major constituent of azurophilic granules of neutrophils. MPO is stored in polymorphonuclear neutrophilic granules and in macrophages, but it is not expressed in lymphocytes, platelets and erythrocytes. MPO catalyzes the formation of hypochlorous acid (HOCl) in the presence of active oxygen (H2O2). Intranuclear MPO may also help to protect DNA against damage resulting from oxygen radicals produced during cell maturation and function. Myeloperoxidase

Permeabilized normal whole blood sample.

Clone: CLB-MPO-1 Isotype: IgG2a Mouse The CLB-MPO-1 antibody recognizes MPO and its enzymatically inactive form. This antibody can be used to detect intracytoplasmic myeloperoxidase in flow cytometry after permeabilization with IntraPrep permeabilization reagent.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC FITC

IM1874

100 tests

Liquid Liquid Liquid Liquid Liquid

CE

IOTest IOTest IOTest IOTest IOTest

98/79/EC

IM1874U IM3455U B36288

2 mL 2 mL

ASR ASR

GMP GMP

PE PE

100 tests

CE

98/79/EC

PC5.5

B23132

0.5 mL

ASR

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 123 Outside US Flow Cytometry Catalog