Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle (G1, S, G2, M phases). It is never expressed by resting (G0) cells. The Ki-67 antigen is a human nuclear protein defined by its reactivity with monoclonal antibody from the Ki-67 clone. Two isoforms of 345 and 395 kDa have been identified by cDNA sequence coding. Deduced amino acid sequence analysis of the Ki-67 antigen confirmed that the cDNA encodes for a nuclear and short-lived protein without any significant homology to known sequences. The Ki-67 antigen is expressed at active phases of the cell cycle (G1, S, G2 and M phases), but it is absent in resting cells (G0 phase). The level of Ki-67 antigen expression varies during cell cycle and it has been correlated to several cell pathways: The Ki-67 decrease pathway is characterized by a declining Ki-67 staining and leads eventually to the exit from the active cell cycle (G0). If cells on this pathway get stimulated by growth factors, they can enter the Ki-67 increase pathway that brings the cells back into S phase. Cells following the Ki-67 stable pathway exhibit a constant intensity of Ki-67 staining during the G1 phase. This pathway is thought to correspond to optimal local growth conditions. The cellular localization of the Ki-67 protein is cell cycle phase dependent. During interphase, the antigen can be exclusively detected within the nucleus, whereas in M phase most of the protein is relocated to the surface of the chromosomes. The antigen is rapidly degraded as the cell enters the non proliferative state. Ki-67

Lysed normal whole blood sample spiked with MOLT4 cell line

Form Clone: Ki-67

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

AF488

B68180

0.5 mL

Liquid

ASR

IOTest

GMP

The Lambda light chain of immunoglobulins is expressed at the surface of a cellular sub- population corresponding to approximately 1/3 of mature B lymphocytes in peripheral blood. This light chain is also found on the surface of a sub-population of immature bone marrow B lymphocytes. λ Chain

DAUDI / NAMALWA Cell line.

Clone: Polyclonal

Isotype: F(ab’) 2

Goat

Form

Size

Format

Part#

Status

Line Quality Standard

PE

C15189 B90416

2 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

PC7

0.5 mL

Clone: Polyclonal

Isotype: F(ab’) 2

Rabbit

Form

Size

Format

Part#

Status

Line Quality Standard

PE PE

A64827 B49174

2 mL

Liquid Liquid

ASR

IOTest IOTest

GMP

100 tests

CE

98/79/EC

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 122 Outside US Flow Cytometry Catalog