Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

Interleukin-2 (IL-2) is a 15-17 kDa cytokine produced and secreted by T cells. The induction of IL -2 secretion is the first step in T cell activation. IL-2 is the major autocrine growth factor for activated T cells and is obligatory for the generation of cytotoxic T cells. IL-2 also promotes the proliferation and the differentiation of NK precursors, B cells and monocytes. Activated CD4+ T cells of the Th1 phenotype produce predominantly IL-2 and Interferon- γ , whereas CD4+ T cells of the Th2 phenotype produce IL-4 and IL-10. IL-2

Activated peripheral blood lymphocytes (PBL).

Form Clone: IL2.39.1

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

FITC

B90436

1 mL

Liquid

ASR

IOTest

GMP

NEW

Interleukin-4 (IL-4) is a monomeric 20 kDa predominantly produced by activated T lymphocytes of the Th2 phenotype, by mast cells and by some antigen-presenting cells. Together with IL-2, IL-4 is an autocrine growth factor for activated T cells and for cytotoxic T cell precursors. IL-4 is a major factor involved in the activation of resting B lymphocytes. Together with IL-13, IL-4 induces isotype switch towards IgE and IgG1 isotypes. Activated CD4+ T cells of the Th1 phenotype produce predominantly IL-2 and Interferon γ , whereas CD4+ T cells of the Th2 phenotype produce IL-4, IL-5, IL6 and IL-10. IL-4

Activated peripheral blood lymphocytes (PBL).

Clone: 4D9 Isotype: IgG1 Mouse The 4D9-PE-conjugated antibody is optimized for flow cytometric detection of intracellular IL-4 in stimulated peripheral mononuclear cells (PBMC) after permeabilizing with formaldehyde / saponin-based IntraPrep Permeabilization Reagent.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM2719U

2 mL

Liquid

ASR

IOTest

GMP

Form Clone: MP4-25D2

Isotype: IgG1 Rat

Size

Format

Part#

Status

Line Quality Standard

PC7

B90441

0.5 mL

Liquid

ASR

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 120 Outside US Flow Cytometry Catalog