Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The anti-IgE mAb binds to circulating IgE as well as to IgE antibodies bound to the low affinity receptor Fc ε RII (expressed mainly on eosinophils, B lymphocytes), or to the high affinity receptor Fc ε RI (expressed on mast cells, basophils, monocytes and monocytes-derived cells). The anti-IgE antibody induces the histamine release from basophils. However, it is known in the literature information that basophils from 10% to 20% of donors completely fail to release histamine in response to anti-IgE, designated anti-IgE "non releasers". IgE (D ε 2)

Lysed normal whole blood sample

Clone: E124.2.8 Isotype: IgG1 Mouse The E124.2.8 monoclonal antibody binds specifically to the D ε 2 constant domain of human Immunoglobulin E (IgE) (D ε 2 is the heat labile region of IgE).

Form

Size

Format

Part#

Status

Line Quality Standard

UNLB

A40174

0.2 mg

Freeze-dried

ASR

-

GMP

The anti-IgM antibody binds to circulating IgM as well as to IgM antibodies bound to the Fc μ R. The Fc μ R is a transmembrane single sialoglycoprotein of 60 kD with O-linked oligosaccharides. It contains an extracellular Ig-like domain homologous to two other IgM-binding receptors (polymeric Ig receptor and Fc α / μ R) but exhibits an exclusive Fc μ -binding specificity. Unlike other FcRs, the major cell types expressing Fc μ R are adaptive immune cells, including B and T lymphocytes. The Fc μ R can be expressed as a cell surface activation antigen throughout the pre-B and B cell stages in differentiation. Receptor expression is not directly linked with IgM production, as both μ - pre-B cells and isotype-switched B cells may express the Fc μ R. The Fc μ R is thus the third member of a family of Fc receptors expressed on B-lineage cells, and its preferential expression on activated B cells suggests a potential role in the response to antigens. IgM

Pre-washed lysed normal whole blood sample.

Clone: SA-DA4

Isotype: IgG1 Mouse

The SA-DA4 monoclonal antibody binds specifically to mu heavy chain of Human Immunoglobulin (IgM).

Form

Size

Format

Part#

Status

Line Quality Standard

PB

B30656 B30655 B30657 B30654

50 tests 50 tests 50 tests 50 tests

Liquid Liquid Liquid Liquid

RUO RUO RUO RUO

IOTest IOTest IOTest IOTest

GMP GMP GMP GMP

FITC

PE

APC

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If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 119 Outside US Flow Cytometry Catalog