Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

Human Interferon- γ (IFN- γ ) is a 20-25 kDa (monomer) pleiotrophic cytokine that is produced by activated T cells and NK cells and is involved in the regulation of almost all immune and inflammatory responses. IFN- γ is involved in the activation, growth and differentiation of T cells, B cells, macrophages, NK cells as well as other cell types. IFN- γ enhances MHC expression on antigen-presenting cells including epithelial, endothelial and connective tissue cell and monocytes cell lines. It also has weak antiviral activity and acts as a macrophage-activating factor (MAF) for tumor cytotoxicity leading to anti-tumor effects. IFN- γ Form Isotype: IgG1 Mouse The FITC- and PE-conjugated forms of the 45.15 antibody are optimized for flow cytometric detection of intracellular IFN- γ in stimulated peripheral blood mononuclear cells (PBMC) after permeabilization with IntraPrep permeabilization reagent. Part# Format Size Status Line Quality Standard PB IOTest ASR B46034 Liquid 0.5 mL GMP FITC IOTest ASR IM2716U Liquid 2 mL GMP PE IOTest ASR IM2717U Liquid 2 mL GMP IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It exists in a transmembrane and a soluble form. IgD is, together with IgM, the first antibody isotypes expressed during B cell ontogeny. Bone marrow B cell precursors acquire surface IgM after assembling heavy (H) and light (L) chain variable region exons from prototypic variable (V), diversity (D) and joining (J) gene segments through an antigen-independent process. After leaving the bone marrow to colonize secondary lymphoid organs, B cells acquire surface IgD of the same specificity as surface IgM through alternative splicing of a pre-messenger RNA comprising V(D)J and both heavy chain constant μ (C μ ) and C δ exons. IgD Clone: 45.15

Activated peripheral blood lymphocytes (PBL).

Pre-washed lysed normal whole blood sample.

Clone: IA6-2

Isotype: IgG2a Mouse

The IA6-2 monoclonal antibody binds specifically to the heavy chain of human IgD.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

B30652 B30653 B30651

50 tests 50 tests 50 tests

Liquid Liquid Liquid

RUO RUO RUO

IOTest IOTest IOTest

GMP GMP GMP

PE

APC

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 118 Outside US Flow Cytometry Catalog