Flow Cytometry catalog - ROW 2019

First page Table of contents Previous page 116 Next page Last page

Single-Color Conjugated Antibodies

HLA-A, -B, and -C are major histocompatibility complex (MHC)-class I antigens. Like other class I molecules (i.e. HLA-E, -F, -G), HLA-A, -B, and -C are heterodimers consisting of a 40 – 45 kDa transmembrane glycoproteic α -chain, noncovalently combined to the invariant β 2- microglobulin. All class I molecules have conserved, monomorphic domains, but are also characterized by their extensive degree of allelic polymorphism. MHC molecules play a central role in the immune response. They are involved in the maturation of T cell repertoire, in the activation of T lymphocytes by presentation of xenogenic peptides or in the allogenic response. HLA-A, -B and -C are “classical” MHC Class I molecules and are expressed on the surface of most nucleated human cell types. HLA-ABC

Lysed normal whole blood sample.

Clone: B9.12.1 Isotype: IgG2a Mouse The B9.12.1 monoclonal antibody recognizes a monomorphic epitope common to HLA-A, -B and -C molecules. B9.12.1 antibody does not recognize unassociated heavy chains.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

IM1838U IM0107

2 mL

Liquid

ASR RUO

IOTest

GMP GMP

UNLB

0.2 mg

Freeze-dried

The HLA (Human Leucocyte Antigen) system is the name given to the major histocompatibility complex (MHC) in man. The MHC complex is located on the short arm of the chromosome 6 and encodes three groups of molecules designated MHC class I, class II and class III. Coded by 5 loci (DM, DO, DP, DQ and DR) of the D locus, HLA class II molecules are also called HLA-DM, HLA-DO, HLA-DP, HLA-DQ and HLA-DR antigens. The HLA class II molecules like class I molecules, are composed of non-covalently associated α / β heteterodimers. On HLA class II, both heavy ( α ) and light ( β ) chain with a molecular weight of 31-33 kDa and 26-29 kDa respectively span the cell membrane. The two immunoglobulin-like domains proximal to the cell membrane ( α 2 and β 2) support the two polymorphic amino-terminal domains ( α 1 and β 1) distal to the membrane which constitute a part of the "antigen presenting site". HLA class II molecules are involved in the regulation of immune response whose expression is limited to antigen presenting cells, i.e. B lymphocytes, monocytes / macrophages, dendritic and Langerhans cells of the skin. HLA-DQ Isotype: IgG2a Mouse The monoclonal antibody SPVL3 recognizes a monomorphic determinant of human class II HLA-DQ molecules The SPVL3 antibody has been used in studies of inhibition of antigen-specific cytolytic T cell clones. Clone: SPVL3

Lysed normal whole blood sample