Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

FMC7 is a transmembrane glycoprotein with a molecular weight of 105 kDa. This antigen was described as a particular conformation of a surface protein encoded by the CD20 gene. FMC7 antibody reactivity to its particular CD20 epitope was found to be dependent of membrane cholesterol level. In circulating blood cells, FMC7 is expressed by a B lymphocyte sub- population. It is not detected on T lymphocytes, NK cells, polymorphonuclear granulocytes, platelets, nor on erythrocytes. The subpopulation of B lymphocytes expressing the FMC7 antgen expresses surface immunoglobulins, thus defining a stage of maturation of the B lymphocytes distinct from FMC7– B lymphocytes. FMC7 ( CD20 )

Lysed normal whole blood sample.

Clone: FMC7 Isotype: IgM Mouse The FMC7 antibody reacts with a subpopulation of peripheral blood B lymphocytes and tonsil B cells, but it does not react with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells.

Form

Size

Format

Part#

Status

Line Quality Standard

PB PB

B08466 B96782 A07791 IM1364U

0.5 mL 50 tests 100 tests

Liquid Liquid Liquid Liquid

ASR

IOTest IOTest IOTest IOTest

GMP

CE CE

98/79/EC 98/79/EC

FITC FITC

1 mL

ASR

GMP

FoxP3 (Forkhead box protein P3, also known as Scurfin, JM2, or IPEX), is the major transcription factor that determines the fate and identity of CD4+ CD25+ Treg cells. It is constitutively expressed in natural Tregs (nTregs) and acts as the master regulator in nTreg development and suppressive function. nTreg (CD4+ CD25+ FoxP3+ Treg cells) are important regulators of almost all immune responses, and they mediate suppressive functions both through the production of cytokines and direct cell–cell contacts. nTreg cells exit in the thymus, circulate and migrate to secondary lymphoid tissues, as well as to inflammatory sites, to exert suppressor activities. FoxP3 expression can also be directly induced in peripheral naïve CD4+ CD25– T cells, forming inducible Treg (iTreg) cells that also suppress immune responses. Diminished Treg cell development and function has been linked to auto-immunity and allergy whereas elevated Treg cell reponses might play a role in undermining anti-infectious and anti- cancer immunity. FoxP3 contains 3 discernible functional domains, a single C2H2 zinc-finger motif, a leucinezipper- like motif, and a C-terminal forkhead domain, thereby exerting its positive and negative function as transcription factor. FoxP3 acts as transcriptional activator for genes that are typically up-regulated in nTregs while it represses transcription of both Th1 and Th2 cytokines in nTregs. FoxP3 can also exert regulatory capacity via protein-protein interactions. FoxP3

Permeabilized normal whole blood sample. Gating on lymphocytes. Dual staining with CD4-FITC and FoxP3- A647.

Clone: 259D

Isotype: IgG1 Mouse

The monoclonal antibody 259D detects endogenous levels of human FoxP3 transcription factor.

Form

Size

Format

Part#

Status

Line Quality Standard

PB PE

B90432 B46031 B46032 B30650

0.5 mL

Liquid Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest IOTest

GMP GMP GMP GMP

1 mL

PC7

0.5 mL 0.5 mL

AF647

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 114 Outside US Flow Cytometry Catalog