Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

CLEC9A is a group V C-type lectin receptor (CTLR) comprised of a single extracellular C-type lectin domain (CTLD) connected to the transmembrane domain by a stalk region and an intracellular cytoplasmic tail. In humans, CLEC9A is expressed on the cell surface of a subset of Dendritic cells (DCs) and a subset of CD14+CD16- Monocytes. In dendritic cells, CLEC9A is selectively expressed on CD141+ (BDCA3+) dendritic cells. As opposed to other DC markers, CLEC9A presents the advantage of being expressed in both human and mouse DCs. Thus, it can serve as a DC marker crossing species barriers and may help in the translation from the mouse DC system to human immunobiology. CD370 ( Clec9A )

K562 Clec9A cell line.

Form Clone: 8F9

Isotype: IgG2a Mouse

Size

Format

Part#

Status

Line Quality Standard

APC

B43291

0.5 mL

Liquid

ASR

IOTest

GMP

In humans, there are two A-type cyclins – an embryonic-specific cyclin A1 and a somatic cyclin A2. Cyclin A1 is only expressed in meiosis and very early embryos, whereas cyclin A2 is present in proliferating somatic cells. Cyclin A2 is a 48 kDa protein composed of 432 amino-acids. Cyclins control the activation of cyclin dependent kinases (CDKs) by associating with them to form a cyclin-CDK complex able to phosphorylate selected proteins and to induce downstream processes. The cyclin A2-CDK2 complex initiates DNA replication and is required for S phase progression. For passage through G2 to M phase, cyclin A2 complexes to the CDK1 (also called CDC2). Cyclin A2 is degraded via the ubiquitin-dependant proteolysis pathway in the early phases of mitosis. Cyclin A2

Human lymphoid cell line (CEM) + Vinblastine.

Clone: 11B2G3

Isotype: IgG1 Mouse

The 11B2G3 antibody reacts with human cyclin A2.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC

A22327 B15092

2 mL 1 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

PE

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 112 Outside US Flow Cytometry Catalog