Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

CD337, also known as NKp30, is a 30-kDa transmembrane glycoprotein which is a NK-specific triggering receptor involved in non-MHC-restricted natural cytotoxicity. NKp30 is strictly expressed by all resting and activated NK cells including the minor CD3–CD56brightCD16– subset. With NKp46 and NKp44 it is one of the three identified NK natural cytotoxicity receptors (NCR). Its surface expression parallels that of NKp46. NKp46dull NK cells are also characterised by a NKp30dull phenotype while NKp46bright NK cells show a NKp30bright phenotype. The NKp30bright or NKp30dull phenotypes are correlated with high or low cytotoxicity, respectively. CD337 is a member of the immunoglobulin superfamily characterized by a single extracellular V-type Ig-like domain. It is associated with a homodimer of ITAM motif- containing CD3 ζ adaptor proteins. These adaptor proteins are not necessary for surface expression of NKp30, but are necessary for signal transduction. In redirected killing assays, Z25-mediated cross linking of NKp30 induces strong NK cell activation. Conversely, Z25- mediated masking of NKp30 inhibits NK cytotoxicity against target cells. More importantly, CD337 represents the major receptor in induced NK-mediated killing of certain target cells, the lysis of which is largely NKp46 / NKp44 independent. Although the CD337 ligand is unknown, studies report NKp30-mediated lysis of immature dendritic cells (iDC). These findings suggest that DCs express one or more unidentified ligands for CD337. CD337 ( NKp30 )

NK92 act. IL2 24h Cell line.

Clone: Z25

Isotype: IgG1 Mouse

The Z25 antibody has been used in flow cytometry to analyze the expression of NKp30 on NK cells.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

IM3709 A66904

1 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

PC5

0.5 mL

The natural killer (NK), T lymphocyte, B lymphocyte antigen (NTB-A) is a 60-kDa transmembrane glycoprotein of the CD2 family expressed on all human resting and activated NK, T and B cells. This antigen is not expressed on monocytes, neutrophils, and basophils but is expressed on eosinophils. The NTB-A antigen, a 331 amino acid protein, is a member of the immunoglobulin (Ig) superfamily. It is characterized, in its 225 amino acid extracellular domain, by one distal V-type domain without disulfide bond and two proximal C2-type domains, and in its 83 amino acid cytoplasmic domain by three tyrosine residues. One tyrosine residue is part of an ITIM motif and two are parts of an ITSM motif (TxYxxV/I). NTB-A is its own ligand through an homophilic binding. NTB-A associates with two adaptor proteins, the SLAM–associated protein (SAP) also known as SH2D1A and the Ewing’s sarcoma activated transcript (EAT) 2. In NK cells, NTB-A acts as an activating coreceptor. CD352 ( NTB-A )

Lysed normal whole blood sample.

Form Clone: MA127

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

A40926

50 tests

Liquid

RUO

IOTest

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 111 Outside US Flow Cytometry Catalog