Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

CD335, also known as NKp46, is a 46-kDa transmembrane glycoprotein which is a NK-specific triggering receptor involved in non-MHC-restricted natural cytotoxicity and considered the prototype of the NK natural cytotoxicity receptors (NCRs). Its expression is restricted to all resting and activated NK cells, including the minor CD3–CD56brightCD16– subset. Although CD335 is highly expressed at the NK-cell surface in the majority of donors, some individuals have a proportion of NK cell (which vary up to 90%) expressing a “dull” NKp46 phenotype. CD335 is a member of the immunoglobulin superfamily characterized by two extracellular C2- type Ig-like domains. It is associated with the ITAM bearing molecules CD3 ζ and Fc ε RI γ . Although this association is likely to be essential for signal transduction via NKp46, CD3 ζ is not required for NKp46 surface expression. NKp46 represents a major activating receptor and plays a central role in the lysis and clearance of HLA class I– cells. The magnitude of lysis correlates with its level of surface expression. CD335 ( NKp46 ) Isotype: IgG1 Mouse BAB 281-mediated masking of NKp46 molecule inhibits NK cytotoxicity versus different target cells. In redirected killing assays, BAB281 binding to NKp46 induces a strong increase of cytolytic activity. Although hemagglutinins of viral origins have been identified as ligands for NKp46 receptor, the natural (non-viral) ligand for NKp46 is unknown. CD336, also known as NKp44, is a 44-kDa transmembrane glycoprotein which is selectively expressed by activated NK cells. NKP44 functions as a triggering receptor involved in non- MHC-restricted natural cytotoxicity by activated NK cells and is one of the NK natural cytotoxicity receptors (NCRs). Structurally, CD336 is a member of the immunoglobulin superfamily characterized by a single extracellular V-type Ig-like domain. It associates with a homodimer of ITAM motif-containing KARAP / DAP12 adaptor proteins. These adaptor proteins were found to be crucial both for the surface expression of NKp44 and for transducing activating signal. CD336 can mediate triggering of NK cell cytotoxicity as in redirected killing assays. Monoclonal antibody Z231-mediated cross-linking of NKp44 in cloned NK cells resulted in strong lysis of target cells and, Z231-mediated masking of NKp44 partially inhibits cytolytic activity. Such inhibition is greatly increased if an anti-CD335, also known as anti-NKp46 (BAB281), is combined with Z231. The CD336 ligand is still undefined. Since activated NK cells selectively express NKp44, it may play a partial role in the enhanced cytotoxicity of IL2-activated compared to those freshly isolated NK cells from peripheral blood. CD336 ( NKp44 ) Clone: BAB281 Part# Format Size Status Line Quality Standard PE IOTest IOTest IOTest ASR ASR ASR IM3711 Liquid Liquid Liquid 1 mL GMP GMP GMP PC5 PC7 A66902 B38703 0.5 mL 0.5 mL

Lysed normal whole blood sample.

Form

Lysed normal whole blood sample.

Form Clone: Z231

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

IM3710 A66903

1 mL

Liquid Liquid

ASR ASR

IOTest IOTest

GMP GMP

PC5

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 110 Outside US Flow Cytometry Catalog