Flow Cytometry catalog - ROW 2019

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Single-Color Conjugated Antibodies

The CD300a, also known as inhibitory receptor protein (IRp60), is a surface molecule of 60 kDa expressed by all human natural killer (NK) cells. The IRp60 gene, located on human chromosome region 17q25 encodes a molecule, highly O- and N-glycosylated, belonging to the immunoglobulin superfamily (Ig-SF) that displays one extracellular Ig-like domain of the V- type, a hydrophobic transmembrane portion and a cytoplasmic tail containing ITIM motifs involved in the inhibitory function. The ligand of the CD300a is still unknown but CD300a does not appear to recognize HLA-class I molecules. Upon cross-linking, IRp60 recruits and activates SH2-containing phosphatases SHP-1 and SHP-2 which switch off the activating signaling cascade by dephosphorylating a variety of tyrosine phosphorylated proteins. Cross-linking of IRp60 by E59.126 monoclonal antibody strongly inhibits the spontaneous cytotoxicity of NK cells as well as the NK-mediated cytolytic activity induced via different non-HLA-specific or HLA-specific activating receptors. CD300a may play a more general role in the regulation of the immune response since it is expressed on other cell types, including T lymphocyte subsets, monocytes and granulocytes. CD300a ( IRp60 )

Lysed normal whole blood sample.

Clone: E59.126

Isotype: IgG1 Mouse

The E59.126 antibody has been used in flow cytometry and Western blotting to characterize IRp60.

Form

Size

Format

Part#

Status

Line Quality Standard

A22328

1 mL

Liquid

ASR

IOTest

GMP

CD305, also called LAIR1 (Leucocyte-associated Ig-like receptor 1), is a 31 kDa type 1 transmembrane molecule with a single N terminal extracellular Ig superfamily C2 domain. The extracellular domain contains one N-glycosylation site. CD305 is expressed on the majority of lymphocytes, NK cells, monocytes, dendritic cells and naïve B cells. It inhibits cell cytotoxicity, cell activation, proliferation and differentiation by phosphorylation of its cytoplasmic ITIM motifs. CD305 ( LAIR1 )