Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD274 antigen is known as Programmed Cell Death 1 ligand 1 (PDCD1LG1 or PD-L1) or B7- H1. It is encoded by a gene located on the human chromosome 9p24. Immune cell function implicates important modulators known as cosignaling receptors. On T cells, the cosignaling receptors belong to either the immunoglobulin (CD28-like) or TNF receptor (TNFR) superfamilies. The CD28 immunoglobulin-superfamily is composed of coinhibitory (CD28, ICOS) and costimulatory receptors. Programmed Death- 1 (PD-1) is one of the three coinhibitory receptors with Cytotoxic T Lymphocyte Antigen 4 (CTLA4) and CD272. CD274 antigen is one of the two ligands, with PD-1 ligand 2 (PD-L2), for PD-1 and belongs to the B7 family. The PD-1 - PD-L1 interaction plays a critical role in regulating T cell activation and tolerance. In particular, it plays a role during development by inhibiting the TCR-mediated positive selection of thymocytes. It has also been shown to be implicated in the regulation of CD4 positive T cells proliferation. CD274 antigen is expressed on immature dendritic cells (iDC), mature dendritic cells (mDC), IFN- γ -treated monocytes, and follicular dendritic cells. CD274 ( PD-L1 ) Isotype: IgG1 Mouse The PD-L1 monoclonal antibody recognizes dendritic cells, T cells and follicular dendritic cells. It has been assigned to the CD274 cluster of differentiation during the 8th HLDA Workshop on Human Leukocyte Differentiation Antigens, held in Adelaide, Australia, in 2004. The CD278 antigen, also known as ICOS molecule (inducible T-cell costimulator, Inducible Costimulator), is a disulfide-linked homodimeric T-cell surface glycoprotein of 55-60 kDa, which belongs to the CD28 and CTLA-4 cell-surface receptor family. The CD278 molecule plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation. While CD278 antigen is not constitutively expressed on naïve peripheral blood T cells, it is highly expressed on unstimulated thymocytes of germinal centers, showing a role in T cell differentiation. Upon activation by antigen presenting cells, T cells (CD4 positive cells and CD8 positive cells) express inducible co-stimulatory receptors, including CD28, CTLA4 and ICOS. The resulting interaction of CD278 (ICOS) with its ligand ICOS-L (B7-like molecule present on B cells) participates to interleukin production, especially IL-10 and IL4. The central role of CD278 in the generation and maintenance of humoral immunity has also been shown to be important. Upon the co-stimulation of T cells through ICOS and CD28, ICOS binds its ligand ICOS-L leading to IgG and IgM secretion as well as IgE production. It is not expressed on resting peripheral T cells, B cells, NK cells, monocytes/macrophages, platelets and granulocytes. CD278 ( ICOS ) Clone: PD-L1 Part# Format Size Status Line Quality Standard PC7 APC IOTest IOTest RUO ASR A78884 B36126 Liquid Liquid 50 tests 0.5 mL GMP GMP

Activated PBL 72h PHA.

Form

Lysed normal whole blood sample.

Form Clone: ISA-3

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

A49080 B36127

100 tests

Liquid Liquid

RUO ASR

IOTest IOTest

GMP GMP

APC

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 105 Outside US Flow Cytometry Catalog