Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

The CD209 antigen, also named dendritic cell (DC)-specific-intracellular adhesion molecule 3 (ICAM-3)-grabbing non-integrin (DC-SIGN), is a 44 kDa type II transmembrane C-type lectin with a single carbohydrate recognition domain (CRD). Like other C-type lectin members, CD209's extracellular structure is able to bind mannose residues in a Ca++ dependent manner. Highly specific to DCs, CD209 plays a key role in the primary immune response as receptor / processor for a variety of ligands, including: ICAM-3: initiation of DC-T cell immunological synapse; ICAM-2: transendothelial DC migration; HIV-1 gp120: capture of HIV-1 and transpresentation to CD4+ T cells; CMV envelope glycoprotein B: capture of CMV; Unknown ligands: capture of Ebola virus, and of Leishmania amastigotes. CD209 is expressed by immature DCs in peripheral tissues, with the noticable exception of Langerhans cells. It is also expressed by DCs in lymphoid tissues, and by certain macrophages. In peripheral blood, a percentage of plasmacytoid DCs (or DC2) may also express CD209. It is totally absent from monocytes, and an observed up-regulation of CD209 expression on monocyte-derived DCs correlates with the down-regulation of CD14. Monocyte-derived macrophages may express CD209, only when cultured in the presence of IL-13. CD209 ( DC-SIGN ) Isotype: IgG1 Mouse The AZND1 monoclonal antibody (mAb) is useful for many purposes, including flow cytometric analysis of CD209- expressing cells, immunohistochemical analysis of CD209 + cells on frozen tissue sections, immunoprecipation of CD209 antigen, and investigation of its functions, due to the blocking or inhibiting properties of AZND1 mAb. The AZND1 mAb to DC-SIGN has been assigned to the CD209 cluster of differentiation during HLDA 7. Clone: AZND1

K562-SIGN Cell line.

Form

Size

Format

Part#

Status

Line Quality Standard

PE

A07407

100 tests

Liquid

RUO

IOTest

GMP

The CD235a antigen, also called Glycophorin A, is a transmembrane, single-pass glycoprotein that contains carbohydrate, mostly in the form of sialic acid. CD235a has a molecular weight of 10 kDa. It is expressed by erythroid precursors and erythrocytes. Glycophorins A carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, and may also serves as receptor for cytokines and pathogens, including the malaria parasite, Plasmodium falciparum and some viruses, including influenza virus and hepatitis A virus. CD235a ( Glycophorin A )

HEL Cell line

Clone: 11E4B-7-6 (KC16) Isotype: IgG1 Mouse The 11E4B-7-6 (KC16) antibody binds to the amino acid sequence 27-39 of glycophorin A and has no reactivity to glycophorin B. It reacts with immature erythrocytes including erythroblasts, proerythroblasts and reticulocytes.

Form

Size

Format

Part#

Status

Line Quality Standard

FITC FITC

IM2212U B49206 A07792 IM2211U A71564 A89314

2 mL

Liquid Liquid Liquid Liquid Liquid Liquid

ASR

IOTest IOTest IOTest IOTest IOTest IOTest

GMP

100 tests 100 tests

CE CE

98/79/EC 98/79/EC

PE PE

2 mL 1 mL

ASR ASR ASR

GMP GMP GMP

PC7

APC-A750

0.5 mL

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 103 Outside US Flow Cytometry Catalog