Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

CD207 (Langerin) is an antigen described as selectively expressed by Langerhans cells (LC), a subset of immature dendritic cells (DC) formed in epidermis and mucosa. Langerin is a 40 kDa glycoprotein predominantly N-glycosylated which contains no disulfide bond. Its expression, restricted to the Langerhans cell lineage, is demonstrated at both the surface membrane and in the cytoplasm. Several lines of evidence indicate that Langerin is a marker specific for the acquisition of the Langerhans cell phenotype. It is down-regulated beyond the LC maturation stage. Langerin is not involved in antigen delivery into the MHC class II pathway but may play a role in antigen capture. Langerin is present only in epidermis and airway epithelia. Langerin is not detected in purified DCs isolated from peripheral blood, lymph nodes or thymus. CD1a+ DCs derived from CD34+ cord blood cell cultures in the presence of GM-CSF and TNF- α express Langerin between day 8 and 12. DCs derived from peripheral blood monocytes cultured with GM-CSF and IL-4 do not express Langerin. CD207 ( Langerin ) Isotype: IgG1 Mouse The DCGM4 antibody stains exclusively immature DCs of the LC lineage. Both intracytoplasmic and surface membrane staining is observed. DCGM4 acts as a ligand for Langerin, triggering rapid endocytosis within 20 minutes at 37 o C. The antibody has been used in intracellular immunofluorescence analyzed in confocal microscopy. The DCGM4 antibody has been used to determine the differentiation of the CD1a + /CD11c + subset of blood DCs into LCs, and in the characterization of DCs infiltrating breast carcinoma tissue. The DC-LAMP antigen (70-90 kDa) is a new member of the LAMP (lysosome-associated membrane protein) family. As other LAMP members such as CD68, DC-LAMP is a type I integral membrane protein of 416 amino acid residues, highly glycosylated (7 N- and some O- glycosylation sites), with 90% of the molecule located in the lumen of lysosomes. DC-LAMP is specifically expressed by those mature dendritic cells (DC) located in T cell areas of lymphoid tissues, which are known as interdigitating dendritic cells (IDC). A direct correlation between the expression of DC-LAMP and that of other maturation markers such as CD86 has been established. DC-LAMP first appears in the MHC Class II compartment, suggesting an important contribution of DC-LAMP to the processing of exogenous antigens. CD208 ( DC-LAMP ) Isotype: IgG1 Mouse In flow cytometry, the 104.G4 antibody shows intracytoplasmic staining of DCs derived either from CD34 + cell cultures activated with TNF α and CD40L, or from monocyte cultures activated with TNF α , LPS and CD40L. Part# Format Size Status Line Quality Standard UNLB - RUO IM3448 Liquid 0.2 mg GMP Clone: DCGM4 Part# Format Size Status Line Quality Standard PE IOTest ASR IM3577 Liquid 2 mL GMP Clone: 104.G4

NIH-LG.13 Cell line.

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NIH-DC.35 cell line.

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If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 102 Outside US Flow Cytometry Catalog