Flow Cytometry catalog - ROW 2019

Single-Color Conjugated Antibodies

CCR7 is G protein-coupled receptors (GPCR) with seven transmembrane receptors. It binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity through their effects on interactions between T cells and dendritic cells. The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B lymphocytes recirculate between B-cell-rich compartments (follicles or B zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis. By bringing together T cells, B cells, and DCs to form functional microenvironments in secondary lymphoid organs, CCR7 has been identified as a major homing receptor and important regulator for initiating an antigen-specific immune response. CD197 ( CCR7 ) CD200 is a highly conserved membrane glycoprotein that belongs to the Ig superfamily (IgSF) containing a two immunoglobulin-like domain (V, C), a single transmembrane and a short cytoplasmic domain. Diverse cell types express CD200 on surface, including thymocytes, B cells, activated T and B cells, dendritic cells, neurons and endothelia. CD200 and CD200R are highly conserved type I membrane glycoproteins, which present N-terminal immunoglobulin- like domains based interaction. While the distribution of CD200 expression is very broad, CD200R is primarily expressed in myeloid and lymphoid cells. They fulfill multiple functions in regulating inflammation interaction by promoting inhibitory activities of the immune system. The interaction between CD200/CD200R results in activation of the intracellular inhibitory pathway with RasGAP recruitment and thus contributes to effector cell inhibition. It was confirmed that the CD200R activation stimulates the differentiation of T cells to the Treg subset, upregulates indoleamine 2,3-dioxygenase activity, modulates cytokine environment from a Th1 to a Th2 pattern, and facilitates an antiinflammatory IL-10 and TGF-b synthesis. CD200/CD200R are required for maintaining selftolerance. Many studies have demonstrated the importance of CD200 in controlling autoimmunity, inflammation, inhibitory activities, hypersensitivity. CD200 Form Clone: G043H7 Isotype: IgG2a Mouse Part# Format Size Status Line Quality Standard PE IOTest IOTest ASR ASR B30632 B46025 Liquid Liquid 1 mL GMP GMP PC7 0.5 mL

Lysed normal whole blood sample.

Lysed normal whole blood sample

Form Clone: OX-104

Isotype: IgG1 Mouse

Size

Format

Part#

Status

Line Quality Standard

PE

B68124 B43299 B92472 B43301

1 mL

Liquid Liquid Liquid Liquid

ASR ASR

IOTest IOTest IOTest IOTest

GMP GMP

PC7 PC7

0.5 mL 50 tests 0.5 mL

CE

98/79/EC

APC-A750

ASR

GMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 100 Outside US Flow Cytometry Catalog