CytoFlex Flow Cytometer Application Notes

TABLE OF CONTENTS

TABLE OF CONTENTS

CytoFLEX Flow Cytometer Title

Page

CytoFLEX Flow Cytometer Platform Brochure................................................................................................................................................................................................................... 1 CytoFLEX Platform 96-well Plate Sample Loaders........................................................................................................................................................................................................... 21

Technical Notes • Successfully importing CytoFLEX CytExpert software data into third party software programs .................................................................................................... 23 • CytoFLEX Research Cytometer Event Rate Settings ........................................................................................................................................................................................... 27 • CytoFLEX Flow Cytometers with CytExpert 2.0 Acquisition and Analysis Software - Support for Compliance with 21 CFR Part 11.................................. 33 • Expanding the Useful Spectrum for Flow Cytometry: Increasing the Number of Parameters Available Without Adding Compensation Complexity...... 37

Application Notes Cell Viability and Vitality

• Viability Assessment of Cell Cultures using the CytoFlex ................................................................................................................................................................................ 54 • Comparing Viability Measurements on Cell Lines Utilizing the CytoFLEX Flow Cytometer ................................................................................................................ 57 • Measuring Proliferation in Leukemia Cell Lines via Carboxyfluorescein Succinimidyl Ester (CFSE) upon Treatment with Cytostatic Concentrations of Cytosine Arabinoside (Ara-C).................................................................................................................................................................................................. 59 • Fully-Automated Cellular Analysis by Flow Cytometry....................................................................................................................................................................................... 63 Immunophenotyping • 6 Color Panel for Staining Human Peripheral Blood Lymphocytes ............................................................................................................................................................... 66 • T Cell Receptor Characterization of PBMCs Using the CytoFlex..................................................................................................................................................................... 68 • Identification of Circulating Myeloid Cell Populations in NLRP3 Null Mice ................................................................................................................................................ 70 • Phenotypic characterizing of human Natural Killer (NK) cell populations in peripheral blood ........................................................................................................... 72 • Identification of NK subsets in mice ........................................................................................................................................................................................................................... 75 • Identification of Natural Killer Cells in Rat Placenta Model of Preeclampsia.............................................................................................................................................. 78 • Competitive Bone Marrow Transplantation in C57/BL6 Mice ........................................................................................................................................................................... 80 • Surface staining of mouse splenocytes and peripheral blood cells .............................................................................................................................................................. 83 • Advanced analysis of human T cell subsets on the CytoFLEX flow cytometer using a 13 color tube based on DuraClone dry reagent technology......... 87 • Characterizing Human CD4+ T cell Populations by Intracellular Cytokine Profile Using Flow Cytometry....................................................................................... 90 • Beadless Absolute Counting - Application of the unique properties of the peristaltic pump fluidic based system for volumetric cell counting............ 95 • 18–Color Human Blood Phenotyping Made Easy with Flow Cytometry....................................................................................................................................................... 102 Functional Assays • Flow Cytometric Quantification to Assess Dorsal Endocytosis........................................................................................................................................................................ 111 • Phagocytosis of Bacteria in a Whole Blood Assay: The Influence of Opsonization on Monocyte and Granulocyte Phagocytosis ..................................... 114 • Single Cell Analysis of Autophagy.............................................................................................................................................................................................................................. 120 • Neutrophil Oxidative Burst Assay: A Dihydrorhodamine (DHR) based testing of Chronic Granulomatous Disease (CGD) with CytoFlex Flow Cytometer ................................................................................................................................................................................................................................... 124 • The unique peristaltic sample delivery system of the CytoFLEX analyzer enables optimized measurements of transient changes in intracellular calcium in cells following agonist activation. ............................................................................................................................................................................... 128 • Flow Cytometric Measurement of CD62P Platelet Activation......................................................................................................................................................................... 132 • Analysis of plant genome sizes using flow cytometry: a case study demonstrating dynamic range and measurement linearity...........................................136 Microbiology • Detecting and counting bacteria with the CytoFLEX research flow cytometer: I-Characterization of a variety of gram-negative bacteria.................. 149 • Detecting and counting bacteria with the CytoFLEX research flow cytometer: II-Characterization of a variety of gram-positive bacteria................... 153 • Counting Eschericia coli Using the CytoFLEX Research Flow Cytometer ................................................................................................................................................. 158 • Flow Cytometric Analysis of Bacterial Protein Aggregation During Expression of Foreign Proteins............................................................................................... 162 • Flow Cytometric Analysis of auto-fluorescent cells found in the marine demosponge Clathria prolifera...................................................................................... 164 • Flow Cytometric Approach to Probiotic Cell Counting and Analysis........................................................................................................................................................... 168