CytoFlex Flow Cytometer Application Notes

Fluorochrome

AF790 Channel

PF840 Channel

AF790

PF-840

Utilizing IR Dyes in Biological Applications Extends Capabilities The addition of the 808 nm laser to the CytoFLEX S series provides additional fluorescent channels not only for use of viability dyes but also bright markers with minimal spectral overlap into traditional channels. Lymphocyte Subset Analysis with Live/dead Discrimination T cells, B cells, and NK cells comprise the lymphocytes and are the mediators of both cellular and humoral immune responses. In healthy individuals, these cells are present in relatively fixed proportions. However, in various diseases the absolute number and proportion will vary. Flow cytometry is the preferred assay for assessing the proportion of cells in these distinct populations. The addition of a parameter to assess cell health and vitality adds an internal sample quality control and allows for dead cells to be removed from the statistical analysis. Dead cells may increase non-specific binding and skew results. A 13 color immunophenotyping panel was designed to detect several major cell populations involved in innate and adaptive immune responses (Figure 7). The gating and population analysis is described in the next paragraph.

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