CytoFlex Flow Cytometer Application Notes

Figure 5. IR dye spill over into other detection channels. VersaComp beads (Beckman Coulter, Part Number B22804) were stained with the recommended amount of single color reagents (Table 1, Beckman Coulter) at room temperature, in the dark for 30 minutes. The samples were washed and then resuspended in 1 mL PBS with Fixative Solution (Beckman Coulter, Part Number A07800). The samples were run on the CytoFLEX S Blue-Red-Violet-Infrared Series and analyzed with CytExpert software v1.2. Each single color is evaluated for its spillover into the AF790 IR channel and the PF840 IR channel. Other than reciprocal spillover by the IR dyes, recognizable by the steep diagonal on the dot plot, all other single colors showed minimal spillover into the AF790 and PF840 IR channels.

Channels

AF790 Stained Beads

PF840 Stained Cells

APC

APC-A700

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