CytoFlex Flow Cytometer Application Notes

Approximate threshold (dual threshold on both FSC and SSC)

• FSC: 10,800

• SSC: 5200

Tips for Data Acquisition and Analysis

The activated platelet sample was acquired first in order to identify CD62P+ platelets.

A gate was drawn on CD62P+ cells on a CD62P histogram, and the gate was assigned a color.

• This color was used to help locate the platelets on the FSC vs SSC plot, and a tight gate (“platelets”) was drawn around the platelets on this plot.

• The CD62P histogram was gated on the “platelet’ events.

Note: The more numerous events in the upper right corner of the FSC vs SSC plot are the leukocytes that will be excluded by this gating.

• The threshold was set such that debris was eliminated without cutting off any platelets.

A media-only control (PBS only) was also acquired to verify the ideal positioning of the threshold to block any background noise.

© 2016 Beckman Coulter Life Sciences. All rights reserved.

Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks used herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at beckman.com FLOW-1911APP08.16