CytoFlex Flow Cytometer Application Notes
Approximate threshold (dual threshold on both FSC and SSC)
• FSC: 10,800
• SSC: 5200
Tips for Data Acquisition and Analysis
The activated platelet sample was acquired first in order to identify CD62P+ platelets.
A gate was drawn on CD62P+ cells on a CD62P histogram, and the gate was assigned a color.
• This color was used to help locate the platelets on the FSC vs SSC plot, and a tight gate (“platelets”) was drawn around the platelets on this plot.
• The CD62P histogram was gated on the “platelet’ events.
Note: The more numerous events in the upper right corner of the FSC vs SSC plot are the leukocytes that will be excluded by this gating.
• The threshold was set such that debris was eliminated without cutting off any platelets.
A media-only control (PBS only) was also acquired to verify the ideal positioning of the threshold to block any background noise.
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