CytoFlex Flow Cytometer Application Notes






Figure 1: Time course of fluorescent bead phagocytosis by REF52 cells, (beads were coated with Collagen I). The uptake of fluorescent beads by REF52 cells was identified through hierarchical gating by first selecting the total cell population (SSC-A vs. FSC-A), then by gating on single cell events (FSC-A vs. FSC-H), finally using the (SSC-A vs. APC-C) parameter to separate negative vs. positive population of cells for bead uptake. A - REF52 cells only, time course:

B - 10min. C - 30min. D - 1h. E - Beads only control.

© 2015 Beckman Coulter Life Sciences. All rights reserved. Beckman Coulter, the stylized logo, Kaluza are trademarks of Beckman Coulter, Inc. and registered in the USPTO. Krome Orange, VersaComp are registered trademarks of Beckman Coulter, Inc. CytoFLEX and CytExpert are trademarks of Xitogen Technologies (Suzhou), Inc., a Beckman Coulter company. All other trademarks are the property of their respective owners. FLOW-1008APP06.15-A.

Made with FlippingBook flipbook maker