CytoFlex Flow Cytometer Application Notes

A

B

C

D

E

Figure 1: Time course of fluorescent bead phagocytosis by REF52 cells, (beads were coated with Collagen I). The uptake of fluorescent beads by REF52 cells was identified through hierarchical gating by first selecting the total cell population (SSC-A vs. FSC-A), then by gating on single cell events (FSC-A vs. FSC-H), finally using the (SSC-A vs. APC-C) parameter to separate negative vs. positive population of cells for bead uptake. A - REF52 cells only, time course:

B - 10min. C - 30min. D - 1h. E - Beads only control.

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