Centrifugation Application Notes

Complete Solutions for Your Specific Exosome Workflow – from Preparation to Analysis

Beckman Coulter provides solutions customized for each step in your specific exosome research workflow – from sample preparation to sample analysis – and can also help improve processes and assist with related challenges.

Sample Preparation

Solution

Step

Jurkat cells grown in log phase density - 1 x 1 0 6 cells/mL

1

Vi-CELL XR

2 Centrifugation to remove cells and debris

Allegra X- 1 5R

3 Ultracentrifugation to remove smaller cellular debris

Optima XPN

This chart provides a sample workflow and the solution for each step.

4 Ultracentrifugation to pellet exosomes

Optima XPN

5 Automated method for density gradient set-up

Biomek 4000Workstation

6 Density gradient ultracentrifugation to isolate exosome from co-purified proteins and other membrane vesicles

Optima XPN

7 Ultracentrifugation to exchange solvent from Iodixanol and sucrose to phosphate buffered saline

Optima MAX-XP

Step Sample Analysis

Solution

8 Size determination and analysis

DelsaMax CORE

Contact your Beckman Coulter Life Sciences Sales Representative for options to improve your processes and to address your specific exosomes workflow:

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