Centrifugation Application Notes

UPSTREAM

DOWNSTREAM

1st Inactivation (Formalin)

Embryonated Chick Eggs

Filtration

Inoculation

Concentration

Zonal Centrifugation

Incubation

Detergent Fragmentation

Candling

Centrifugation

Chilling Overnight

2nd Inactivation (Formalin)

UF Diafiltration

Harvest (Pool Allantoic Fluid)

Sterile Filtration

Clarification

Final Concentrate Product

Fig. 1. Typical workflow for egg-based influenza vaccine manufacturing. 1