Biomek iSeries

Modular workflow enables flexible throughput and scheduling of your day

40-120ng of Sheared gDNA

Day 1

A-Tailing, Adapter Ligation, and Cleanup

cDNA Synthesis and Cleanup

First Hybridization

40-85ng of RNA

Index PCR

Day 2

Cleanup Amplified Libraries

Second Capture and Libraies Amplification Setup

Normalize Libraries

Prepare for Sequencing

Second Hybridization

First Capture

Automated Process

Quantify Libraries

Instrument Setup

Key

Mamual Process

Start/Stop Point

Figure 1. Automated Illumina TruSight Tumor 170 workflow

Table 1. Day 1 Time Estimates

8 RNA and 8 DNA

16 RNA and 16 DNA

Day 1

Offline Time Instrument Time Offline Time Instrument Time

Denature and Anneal RNA, First Strand Synthesis, Second Strand cDNA Synthesis, and cDNA Cleanup

15 mins

2 hr

25 min

2 hr, 15 min

DNA Input Preparation and Fragmentation (Manual)

*1 hr, 30 min -

2 hr

-

End Repair and A-Tailing, Ligate Adapters, and Clean Up Ligation

*15 min

2 hr, 40 min

*15 min

3 hr

Indexing PCR (Manual)

45 min

-

50 min

-

First Hybridization (Manual)

*Overnight

-

*Overnight

-

Day 1 Offline Time

1 hr

1hr, 15 min

Day 1 Instrument Time

4 hr, 40 min

5 hr, 15 min

Day 1 Total Time

5 hr, 40 min

6 hr, 30 min

* Steps that can be run in parallel with other on-going processes are not included in total time count.

Table 2. Day 2 Time Estimates

8 RNA and 8 DNA

16 RNA and 16 DNA

Day 2

Offline Time Instrument Time Offline Time Instrument Time

First Capture

15 min

1hr, 45 min

15 min

2 hr

Min: 1 hr, 30 *Max: 4 hr

Min: 1 hr, 30 *Max: 4 hr

Second Hybridization (Manual)

-

-

Second Capture and Amplify Enriched Libraries Set Up

*15 min

1 hr, 20 min

*15 min

1 hr, 35 min

Amplify Enriched Libraries (Manual)

50 min

-

50 min

-

Clean Up Amplified Libraries

*15 min

40 min

*15 min

55 min

Library Quantification

*20 min

-

*20 min

-

Normalize Libraries

*15 min

45 min

*15 min

1 hr

Sequencing Set Up (Manual)

20 min

-

25 min

-

Day 2 Offline Time

2 hr, 55 min

3 hr

Day 2 Instrument Time

4 hr, 30 min

5 hr, 30 min

Day 2 Total Time

7 hr, 25 min

8hr, 30 min

* Steps that can be run in parallel with other on-going processes are not included in total time count.

Learn more

Contact Beckman Coulter at 1-800-742-2345

Biomek Automated Workstations are not intended or validated for use in the diagnosis of disease or other conditions © 2017 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks used herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. For Beckman Coulter’s worldwide office locations and phone numbers, please visit “Contact Us” at beckman.com AAG-3266FLY12.17