2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD180

CD180 is a type 1 membrane protein consisting of extracellular leucinerich repeats (LRR) and a short cytoplasmic tail. It regulates B cell recognition of lipopolysaccharide. The extracellular LRR is associated with a molecule called MD-1 and forms the cell surface receptor complex RP105/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). RP105/MD-1, working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane of constituent of Gram-negative bacteria. The extracellular domain of CD180 contains 22 LRR, tandem repeats of leucine-rich motif (LMR). This LMR is a 24 -28 amino acid sequence in which leucines are characteristically positioned. The ligation of CD180 on B cells by a monoclonal antibody induces activation that leads to upregulation of costimultory molecules, CD80 and CD86, and an increased cell size. CD180 is expressed on mantle zone B cells and marginal zone B cells, but weakly or negative on germinal center B cells. CD180 is also expressed on peripheral blood monocytes and dendritic cells.

Lysed normal whole blood sample.

Clone: MHR73-11 (IgG1 Mouse) The monoclonal antibody MHR73-11 was generated against human RP105 complexed with mouse MD-1 and recognizes the CD180 protein.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

B68148

1 mL

Liquid

ASR

IOTest

cGMP

CD183, also called CXCR3, is a 41 kDa transmembrane G protein-coupled CXC receptor, belonging to the family of chemokine receptors. It has selectivity for IP10, Mig and I-TAC chemokines. Cellular production of CD183 ligands is induced by interferon, suggesting an important role of CD183 in Th1-type inflammatory processes. CD183 signaling induces chemotactic migration in inflammation-associated effector T cells, involving integrin activation and cytoskeletal changes. CD183 is expressed on T cells (Th1), NK cells, dendritic cells, mast cells, eosinophils. CD183 ( CXCR3 )

Lysed normal whole blood sample.

Clone: G025H7 (IgG1 Mouse)

Form

Format

Part#

Status

Line

Quality Standard

Size

AF488

B68144

0.5 mL

Liquid

ASR

IOTest

cGMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 97 Outside US Flow Cytometry Catalog