2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

The CD161 antigen is also designated as the NKR-P1A molecule. CD161 is a type II integral membrane protein expressed as a monomer of 44 kDa or as a disulphide- linked homodimer of 80 kDa. The carboxy-terminal extracellular domain is homologous to Ca++-dependent (C-type) lectin superfamily. CD161 is an activatory NK receptor, but in contrast with other NK receptors such as KIR (p58, p70, p140) and CD94/NKG2, CD161-mediated activation is not HLA-dependent. CD161 is expressed on all NK cells and on sub-populations of T lymphocytes. CD161 ( NKR-P1A )

Lysed normal whole blood sample.

Clone: 191B8 (IgG2a Mouse) The 191B8 antibody partially blocks the spontaneous NK cytotoxicity in the P815 model.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

IM3450 B30631 B30630

1 mL

Liquid Liquid Liquid

ASR ASR ASR

IOTest IOTest IOTest

cGMP cGMP cGMP

PC7

0.5 mL 0.5 mL

APC-A750

The CD166 antigen, also known as activated leukocyte cell adhesion molecule (ALCAM), is a single chain type 1 transmembrane glycoprotein, containing 10 potential N-linked glycosylation sites, and belonging to the immunoglobulin superfamily (IgSF). It shows a 100 – 105 kDa molecular weight, and is composed of five extracytoplasmic Ig-like domains linked to a 32-amino acid cytoplasmic tail through a short membrane-spanning region. The CD166 antigen is an adhesion molecule that binds to the CD6 molecule, or forms ALCAM-ALCAM homophilic interactions. Studies suggest that CD166 molecules can oligomerize at the site of cell- cell contact via their three C2-type Ig-like proximal domains. The CD166 molecule appears to be a non-lineage restricted molecule, expressed on a variety of cell types, including selected hematopoietic cells, endothelial cells, cortical and medullar thymic epithelial cells, bone marrow mesenchymal stem cells, fibroblasts, and hepatocytes. In peripheral blood, ALCAM is expressed on activated T- and B-cell subsets, on monocytes, on circulating dendritic cells, and weakly on granulocytes. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration. In the thymus, the interaction between the CD6 molecule, as expressed on CD6+ thymocytes, and the CD166 molecule expressed on thymic epithelial cells may play a role in T cell development. Other studies suggest that the CD166 adhesion molecule is involved in embryonic hematopoiesis, as well as in hemangioblast differentiation and capillary tube formation. CD166 ( ALCAM )

Lysed normal whole blood sample.

Clone: 3A6 (IgG1 Mouse) The 3A6 antibodyblocksthe bindingof the CD6ligandto its CD166receptoras seenon adhesionexperimentsof CD6-expressingCOScellsto thymicepithelial cells. The 3A6 antibody was clustered to the CD166 during HLDA 6.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

A22361

2 mL

Liquid

ASR

IOTest

cGMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 96 Outside US Flow Cytometry Catalog