2020 Outside US Flow Cytometry Catalog

Single-Color Conjugated Antibodies

CD159a (NKG2a) is a 40 kDa (reduced), 75 kDa (unreduced) type II transmembrane protein associated with CD94 to form a disulphide-linked heterodimer. CD159a is a member of the Ca++-dependent (C-type) lectin family. The CD94/NKG2A dimer is an inhibitory NK receptor for MHC Class I, with a broader specificity than Killer cell Immunoglobin (Ig)-like Receptors (KIR). The intracellular portion of CD159a (NKG2A) contains two characteristic ITIM motifs (Immunoreceptor tyrosine based inhibition motif) involved in the transduction of the inhibitory signal. The CD159a (NKG2A) antigen is always co-expressed with CD94. The CD94/NKG2A complex is detectable in sub-populations of NK cells, T cells and thymocytes. CD159a ( NKG2a )

Lysed normal whole blood sample.

Clone: Z199 (IgG2b Mouse) The Z199antibodyreactsspecificallywith CD94

bright NK cells expressingthe inhibitoryformof the CD94molecules.It doesnot stainthe activatorformof CD94

whichcorrespondsto the associationof CD94withotherproductsof theNKG2agenefamily.Theantibodyrestoresthe abilityof CD94 bright NK clonesto lyseHLA class I targets. Z199 cross reacts with: Cynomolgus Monkey, Indian Rhesus, Pigtailed Macaque, African Green, Chimpanzee, Olive Baboon, Common Marmoset.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

IM3291U B10246 A60797 IM2750

1 mL

Liquid Liquid Liquid

ASR ASR ASR ASR

IOTest IOTest IOTest

cGMP cGMP cGMP cGMP

PC7 APC

0.5 mL 0.5 mL 0.2 mg

UNLB

Freeze-dried

-

CD160

The CD160 molecule (BY55 antigen) is a 27-kDa, glycosyl phosphatidylinositol (GPI)- anchored glycoprotein of 181 amino acids, with a single immunoglobulin (Ig)-like domain. The molecule contains two potential sites for N-glycosylation and is rich in cysteine (6 residues in the mature polypeptide), indicating an important capacity for forming intra- and inter-chain disulfide bonds, which likely support the disulfide-linked multimeric (80 kDa) expression at the cell surface. Its expression is highly restricted to circulating cytotoxic T and NK cells, and in tissues, to intestinal intraepithelial lymphocytes, as revealed by CD160 mRNA blot analysis and various phenotyping studies. In peripheral blood, CD160+ cells consist of a majority of CD3– TCR γδ + NK cells, which is significantly reduced in HIV+ individuals, and of a minority of CD3+ CD8bright T lymphocytes, which is significantly increased in HIV+ individuals. In bone marrow, CD160+ cells, though less abundant than in adult peripheral blood, also consist of the two populations observed in peripheral blood. In cord blood, CD160+ cells consist of a unique CD3– TCR– NK cell population, 80% of these cells being CD56+. CD160 is a ligand for classical and non-classical MHC-class I molecules. The ligation of MHC-class I by CD160 provides a costimulatory signal restricted to peripheral blood activated T cells, suggesting a pathway being additional and/or alternative to the CD28 costimulation mechanism. This pathway may be relevant in memory T cells that lack CD28, such as the intestinal intraepithelial lymphocytes.

YT Cell line.

Clone: BY55 (IgM Mouse) TheBY55antibodywasinitiallyreportedto reactwitha proteinstructureof 80 kDaexclusivelyexpressedby circulatingcytotoxiclymphocytes.It doesnot block the binding of the CD160 molecule to MHC-class I molecules. BY55 cross reacts with: Sooty Mangabey.

Form

Format

Part#

Status

Line

Quality Standard

Size

PE

IM3657

2 mL

Liquid

ASR

IOTest

cGMP

If you require a different antibody fluorochrome combination, please refer to the Custom Design Services on page 14 or contact us via: cds.support@beckman.com ANTIBODIES & KITS 95 Outside US Flow Cytometry Catalog